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COL17A1 Protein (AA 524-1497) (rho-1D4 tag)

Crystallography grade COL17A1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3091906
  • Target See all COL17A1 Proteins
    COL17A1 (Collagen, Type XVII, alpha 1 (COL17A1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 524-1497
    Origin
    • 4
    • 4
    Human
    Source
    • 8
    Insect Cells
    Purification tag / Conjugate
    This COL17A1 protein is labelled with rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    LQGMAPAAGA DLDKIGLHSD SQEELWMFVR KKLMMEQENG NLRGSPGPKG DMGSPGPKGD RGFPGTPGIP GPLGHPGPQG PKGQKGSVGD PGMEGPMGQR GREGPMGPRG EAGPPGSGEK GERGAAGEPG PHGPPGVPGS VGPKGSSGSP GPQGPPGPVG LQGLRGEVGL PGVKGDKGPM GPPGPKGDQG EKGPRGLTGE PGMRGLPGAV GEPGAKGAMG PAGPDGHQGP RGEQGLTGMP GIRGPPGPSG DPGKPGLTGP QGPQGLPGTP GRPGIKGEPG APGKIVTSEG SSMLTVPGPP GPPGAMGPPG PPGAPGPAGP AGLPGHQEVL NLQGPPGPPG PRGPPGPSIP GPPGPRGPPG EGLPGPPGPP GSFLSNSETF LSGPPGPPGP PGPKGDQGPP GPRGHQGEQG LPGFSTSGSS SFGLNLQGPP GPPGPQGPKG DKGDPGVPGA LGIPSGPSEG GSSSTMYVSG PPGPPGPPGP PGSISSSGQE IQQYISEYMQ SDSIRSYLSG VQGPPGPPGP PGPVTTITGE TFDYSELASH VVSYLRTSGY GVSLFSSSIS SEDILAVLQR DDVRQYLRQY LMGPRGPPGP PGASGDGSLL SLDYAELSSR ILSYMSSSGI SIGLPGPPGP PGLPGTSYEE LLSLLRGSEF RGIVGPPGPP GPPGIPGNVW SSISVEDLSS YLHTAGLSFI PGPPGPPGPP GPRGPPGVSG ALATYAAENS DSFRSELISY LTSPDVRSFI VGPPGPPGPQ GPPGDSRLLS TDASHSRGSS SSSHSSSVRR GSSYSSSMST GGGGAGSLGA GGAFGEAAGD RGPYGTDIGP GGGYGAAAEG GMYAGNGGLL GADFAGDLDY NELAVRVSES MQRQGLLQGM AYTVQGPPGQ PGPQGPPGIS KVFSAYSNVT ADLMDFFQTY GAIQGPPGQK GEMGTPGPKG DRGPAGPPGH PGPPGPRGHK GEKGDKGDQV YAGRRRRRSI AVKP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human COL17A1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product COL17A1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    COL17A1 (Collagen, Type XVII, alpha 1 (COL17A1))
    Alternative Name
    COL17A1 (COL17A1 Products)
    Synonyms
    col17a1 Protein, fk89a06 Protein, si:ch211-223p8.6 Protein, wu:fk89a06 Protein, COL17A1 Protein, BA16H23.2 Protein, BP180 Protein, BPA-2 Protein, BPAG2 Protein, LAD-1 Protein, Bpag Protein, Bpag2 Protein, collagen type XVII alpha 1 chain Protein, collagen, type XVII, alpha 1b Protein, collagen, type XVII, alpha 1 Protein, Col17a1 Protein, col17a1b Protein, COL17A1 Protein
    Background
    May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane., The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.
    Molecular Weight
    96.5 kDa Including tag.
    UniProt
    Q9UMD9
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