DERL1 Protein (AA 2-251) (rho-1D4 tag)

Catalog No. ABIN3092140

Product Details

Target: DERL1 (Der1-Like Domain Family, Member 1 (DERL1))

Protein Type: Recombinant

Protein Characteristics: AA 2-251

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This DERL1 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: SDIGDWFRSI PAITRYWFAA TVAVPLVGKL GLISPAYLFL WPEAFLYRFQ IWRPITATFY FPVGPGTGFL YLVNLYFLYQ YSTRLETGAF DGRPADYLFM LLFNWICIVI TGLAMDMQLL MIPLIMSVLY VWAQLNRDMI VSFWFGTRFK ACYLPWVILG FNYIIGGSVI NELIGNLVGH LYFFLMFRYP MDLGGRNFLS TPQFLYRWLP SRRGGVSGFG VPPASMRRAA DQNGGGGRHN WGQGFRLGDQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human DERL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DERL1 (Der1-Like Domain Family, Member 1 (DERL1))

Alternative Name: DERL1 (DERL1 Products)

Synonyms: CG10908 Protein, Dmel\\CG10908 Protein, DDBDRAFT_0188135 Protein, DDBDRAFT_0266772 Protein, DDB_0188135 Protein, DDB_0266772 Protein, MGC84200 Protein, wu:fb09d07 Protein, wu:fb94d05 Protein, wu:fj92g12 Protein, zgc:55637 Protein, 1110021N07Rik Protein, AI195141 Protein, AW551338 Protein, Derlin-1 Protein, derlin-1 Protein, DER-1 Protein, DER1 Protein, RGD1311835 Protein, DERLIN-1 Protein, F17A13.150 Protein, F17A13_150 Protein, Derlin-1 Protein, derlin-1 Protein, derlin-1.1 Protein, hypothetical protein Protein, Der1-like domain family, member 1 Protein, derlin 1 Protein, derlin 1 L homeolog Protein, DERLIN-1 Protein, Der-1 Protein, EDI_023440 Protein, PTRG_01331 Protein, derl1 Protein, DERL1 Protein, derl1.L Protein, Derl1 Protein, DER1 Protein

Background: Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and the degradation substrate. In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein. {ECO:0000269|PubMed:15215855, ECO:0000269|PubMed:15215856}.

Molecular Weight: 29.8 kDa Including tag.

UniProt: Q9BUN8

Pathways: ER-Nucleus Signaling