DHX29 Protein (AA 1-1369) (Strep Tag)

Catalog No. ABIN3092154

Product Details

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Protein Type: Recombinant

Protein Characteristics: AA 1-1369

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DHX29 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MGGKNKKHKA PAAAVVRAAV SASRAKSAEA GIAGEAQSKK PVSRPATAAA AAAGSREPRV KQGPKIYSFN STNDSSGPAN LDKSILKVVI NNKLEQRIIG VINEHKKQNN DKGMISGRLT AKKLQDLYMA LQAFSFKTKD IEDAMTNTLL YGGDLHSALD WLCLNLSDDA LPEGFSQEFE EQQPKSRPKF QSPQIQATIS PPLQPKTKTY EEDPKSKPKK EEKNMEVNMK EWILRYAEQQ NEEEKNENSK SLEEEEKFDP NERYLHLAAK LLDAKEQAAT FKLEKNKQGQ KEAQEKIRKF QREMETLEDH PVFNPAMKIS HQQNERKKPP VATEGESALN FNLFEKSAAA TEEEKDKKKE PHDVRNFDYT ARSWTGKSPK QFLIDWVRKN LPKSPNPSFE KVPVGRYWKC RVRVIKSEDD VLVVCPTILT EDGMQAQHLG ATLALYRLVK GQSVHQLLPP TYRDVWLEWS DAEKKREELN KMETNKPRDL FIAKLLNKLK QQQQQQQQHS ENKRENSEDP EESWENLVSD EDFSALSLES ANVEDLEPVR NLFRKLQSTP KYQKLLKERQ QLPVFKHRDS IVETLKRHRV VVVAGETGSG KSTQVPHFLL EDLLLNEWEA SKCNIVCTQP RRISAVSLAN RVCDELGCEN GPGGRNSLCG YQIRMESRAC ESTRLLYCTT GVLLRKLQED GLLSNVSHVI VDEVHERSVQ SDFLLIILKE ILQKRSDLHL ILMSATVDSE KFSTYFTHCP ILRISGRSYP VEVFHLEDII EETGFVLEKD SEYCQKFLEE EEEVTINVTS KAGGIKKYQE YIPVQTGAHA DLNPFYQKYS SRTQHAILYM NPHKINLDLI LELLAYLDKS PQFRNIEGAV LIFLPGLAHI QQLYDLLSND RRFYSERYKV IALHSILSTQ DQAAAFTLPP PGVRKIVLAT NIAETGITIP DVVFVIDTGR TKENKYHESS QMSSLVETFV SKASALQRQG RAGRVRDGFC FRMYTRERFE GFMDYSVPEI LRVPLEELCL HIMKCNLGSP EDFLSKALDP PQLQVISNAM NLLRKIGACE LNEPKLTPLG QHLAALPVNV KIGKMLIFGA IFGCLDPVAT LAAVMTEKSP FTTPIGRKDE ADLAKSALAM ADSDHLTIYN AYLGWKKARQ EGGYRSEITY CRRNFLNRTS LLTLEDVKQE LIKLVKAAGF SSSTTSTSWE GNRASQTLSF QEIALLKAVL VAGLYDNVGK IIYTKSVDVT EKLACIVETA QGKAQVHPSS VNRDLQTHGW LLYQEKIRYA RVYLRETTLI TPFPVLLFGG DIEVQHRERL LSIDGWIYFQ APVKIAVIFK QLRVLIDSVL RKKLENPKMS LENDKILQII TELIKTENN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))

Alternative Name: DHX29 (DHX29 Products)

Synonyms: DDX29 Protein, 3732415M03 Protein, AU043276 Protein, Ddxx Protein, E130202M19Rik Protein, DExH-box helicase 29 Protein, DEAH (Asp-Glu-Ala-His) box polypeptide 29 Protein, DEAH-box helicase 29 L homeolog Protein, DEAH-box helicase 29 Protein, DHX29 Protein, Dhx29 Protein, dhx29.L Protein, dhx29 Protein

Background: ATP-dependent RNA helicase DHX29 (EC 3.6.4.13) (DEAH box protein 29) (Nucleic acid helicase DDXx),FUNCTION: ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. {ECO:0000255|HAMAP-Rule:MF_03068, ECO:0000269|PubMed:19109895, ECO:0000269|PubMed:23706745}.

Molecular Weight: 155.2 kDa

UniProt: Q7Z478