DSCAM Protein (AA 1617-2012) (His tag)

Catalog No. ABIN3092182

Product Details

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Protein Type: Recombinant

Protein Characteristics: AA 1617-2012

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This DSCAM protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: RRRRREQRLK RLRDAKSLAE MLMSKNTRTS DTLSKQQQTL RMHIDIPRAQ LLIEERDTME TIDDRSTVLL TDADFGEAAK QKSLTVTHTV HYQSVSQATG PLVDVSDARP GTNPTTRRNA KAGPTARNRY ASQWTLNRPH PTISAHTLTT DWRLPTPRAA GSVDKESDSY SVSPSQDTDR ARSSMVSTES ASSTYEELAR AYEHAKMEEQ LRHAKFTITE CFISDTSSEQ LTAGTNEYTD SLTSSTPSES GICRFTASPP KPQDGGRVMN MAVPKAHRPG DLIHLPPYLR MDFLLNRGGP GTSRDLSLGQ ACLEPQKSRT LKRPTVLEPI PMEAASSASS TREGQSWQPG AVATLPQREG AELGQAAKMS SSQESLLDSR GHLKGNNPYA KSYTLV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human DSCAM Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Alternative Name: DSCAM (DSCAM Products)

Synonyms: CHD2-42 Protein, CHD2-52 Protein, Dscam Protein, DSCAM Protein, chd2-42 Protein, chd2-52 Protein, dscam Protein, GB15141 Protein, 4932410A21Rik Protein, 43Bc Protein, CG17800 Protein, CT39257 Protein, DScam Protein, DmDscam Protein, Dm_2R:13612 Protein, Dmel\\CG17800 Protein, Dscam-hv Protein, Dscam1 Protein, FBgn0033159 Protein, Neu1 Protein, dScam Protein, l(2)05518 Protein, l(2)43Bc Protein, p270 Protein, DS cell adhesion molecule Protein, Down syndrome cell adhesion molecule Protein, Down syndrome cell adhesion molecule a Protein, Down syndrome cell adhesion molecule 1 Protein, Down syndrome cell adhesion molecule-like protein Dscam2 Protein, down syndrome cell adhesion molecule Protein, Down syndrome cell adhesion molecule L homeolog Protein, DSCAM Protein, Dscam Protein, dscam Protein, dscama Protein, Dscam1 Protein, LOC5573626 Protein, CpipJ_CPIJ006173 Protein, dscam.L Protein

Background: Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies (PubMed:10925149). Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding (PubMed:18585357, PubMed:19196994). Enhances netrin-induced phosphorylation of PAK1 and FYN (PubMed:15169762). Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38 (PubMed:18585357, PubMed:19196994). Adhesion molecule that promotes lamina-specific synaptic connections in the retina: expressed in specific subsets of interneurons and retinal ganglion cells (RGCs) and promotes synaptic connectivity via homophilic interactions (By similarity). {ECO:0000250|UniProtKB:F1NY98, ECO:0000269|PubMed:10925149, ECO:0000269|PubMed:15169762, ECO:0000269|PubMed:18585357, ECO:0000269|PubMed:19196994}.

Molecular Weight: 44.7 kDa Including tag.

UniProt: O60469

Pathways: Regulation of Cell Size