ERO1LB Protein (AA 34-467) (His tag)

Catalog No. ABIN3092507

Product Details

Target: ERO1LB (ERO1-Like beta (ERO1LB))

Protein Type: Recombinant

Protein Characteristics: AA 34-467

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This ERO1LB protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: QVTGVLDDCL CDIDSIDNFN TYKIFPKIKK LQERDYFRYY KVNLKRPCPF WAEDGHCSIK DCHVEPCPES KIPVGIKAGH SNKYLKMANN TKELEDCEQA NKLGAINSTL SNQSKEAFID WARYDDSRDH FCELDDERSP AAQYVDLLLN PERYTGYKGT SAWRVWNSIY EENCFKPRSV YRPLNPLAPS RGEDDGESFY TWLEGLCLEK RVFYKLISGL HASINLHLCA NYLLEETWGK PSWGPNIKEF KHRFDPVETK GEGPRRLKNL YFLYLIELRA LSKVAPYFER SIVDLYTGNA EEDADTKTLL LNIFQDTKSF PMHFDEKSMF AGDKKGAKSL KEEFRLHFKN ISRIMDCVGC DKCRLWGKLQ TQGLGTALKI LFSEKEIQKL PENSPSKGFQ LTRQEIVALL NAFGRLSTSI RDLQNFKVLL QHSR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ERO1B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ERO1LB (ERO1-Like beta (ERO1LB))

Alternative Name: ERO1B (ERO1LB Products)

Synonyms: RGD1563548 Protein, 1300013B24Rik Protein, 1700065B09Rik Protein, AI447560 Protein, ero1-beta Protein, endoplasmic reticulum oxidoreductase 1 beta Protein, ERO1-like beta (S. cerevisiae) Protein, Ero1b Protein, ERO1B Protein, Ero1lb Protein

Background: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50 % of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10 %). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis. {ECO:0000269|PubMed:11707400, ECO:0000269|PubMed:21091435}.

Molecular Weight: 51.2 kDa Including tag.

UniProt: Q86YB8

Pathways: SARS-CoV-2 Protein Interactome