FCHO1 Protein (AA 1-889) (Strep Tag)

Catalog No. ABIN3092572

Product Details

Target: FCHO1 (FCH Domain Only 1 (FCHO1))

Protein Type: Recombinant

Protein Characteristics: AA 1-889

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FCHO1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSYFGEHFWG EKNHGFEVLY HSVKQGPIST KELADFIRER ATIEETYSKA MAKLSKLASN GTPMGTFAPL WEVFRVSSDK LALCHLELTR KLQDLIKDVL RYGEEQLKTH KKCKEEVVST LDAVQVLSGV SQLLPKSREN YLNRCMDQER LRRESTSQKE MDKAETKTKK AAESLRRSVE KYNSARADFE QKMLDSALRF QAMEETHLRH MKALLGSYAH SVEDTHVQIG QVHEEFKQNI ENVSVEMLLR KFAESKGTGR EKPGPLDFEA YSAAALQEAM KRLRGAKAFR LPGLSRRERE PEPPAAVDFL EPDSGTCPEV DEEGFTVRPD VTQNSTAEPS RFSSSDSDFD DEEPRKFYVH IKPAPARAPA CSPEAAAAQL RATAGSLILP PGPGGTMKRH SSRDAAGKPQ RPRSAPRTSS CAERLQSEEQ VSKNLFGPPL ESAFDHEDFT GSSSLGFTSS PSPFSSSSPE NVEDSGLDSP SHAAPGPSPD SWVPRPGTPQ SPPSCRAPPP EARGIRAPPL PDSPQPLASS PGPWGLEALA GGDLMPAPAD PTAREGLAAP PRRLRSRKVS CPLTRSNGDL SRSLSPSPLG SSAASTALER PSFLSQTGHG VSRGPSPVVL GSQDALPIAT AFTEYVHAYF RGHSPSCLAR VTGELTMTFP AGIVRVFSGT PPPPVLSFRL VHTTAIEHFQ PNADLLFSDP SQSDPETKDF WLNMAALTEA LQRQAEQNPT ASYYNVVLLR YQFSRPGPQS VPLQLSAHWQ CGATLTQVSV EYGYRPGATA VPTPLTNVQI LLPVGEPVTN VRLQPAATWN LEEKRLTWRL PDVSEAGGSG RLSASWEPLS GPSTPSPVAA QFTSEGTTLS GVDLELVGSG YRMSLVKRRF ATGMYLVSC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FCHO1 (FCH Domain Only 1 (FCHO1))

Alternative Name: FCHO1 (FCHO1 Products)

Synonyms: 3322402E17Rik Protein, N28152 Protein, FCH domain only 1 Protein, FCH domain only 1 L homeolog Protein, FCH domain only protein 1 Protein, fcho1 Protein, fcho1.L Protein, LOC100462182 Protein, FCHO1 Protein, Fcho1 Protein

Background: F-BAR domain only protein 1,FUNCTION: Functions in an early step of clathrin-mediated endocytosis (PubMed:30822429). Has both a membrane binding/bending activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. May regulate Bmp signaling by regulating clathrin-mediated endocytosis of Bmp receptors. Involved in the regulation of T-cell poliferation and activation (PubMed:30822429, PubMed:32098969). Affects TCR clustering upon receptor triggering and modulates its internalisation, playing a role in TCR-dependent T-cell activation (PubMed:32098969). {ECO:0000269|PubMed:20448150, ECO:0000269|PubMed:30822429, ECO:0000269|PubMed:32098969}.

Molecular Weight: 96.9 kDa

UniProt: O14526