FBXO9 Protein (AA 1-447) (Strep Tag)

Catalog No. ABIN3092582

Product Details

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Protein Type: Recombinant

Protein Characteristics: AA 1-447

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FBXO9 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPDIIWVFPP QAEAEEDCHS DTVRADDDEE NESPAETDLQ AQLQMFRAQW MFELAPGVSS SNLENRPCRA ARGSLQKTSA DTKGKQEQAK EEKARELFLK AVEEEQNGAL YEAIKFYRRA MQLVPDIEFK ITYTRSPDGD GVGNSYIEDN DDDSKMADLL SYFQQQLTFQ ESVLKLCQPE LESSQIHISV LPMEVLMYIF RWVVSSDLDL RSLEQLSLVC RGFYICARDP EIWRLACLKV WGRSCIKLVP YTSWREMFLE RPRVRFDGVY ISKTTYIRQG EQSLDGFYRA WHQVEYYRYI RFFPDGHVMM LTTPEEPQSI VPRLRTRNTR TDAILLGHYR LSQDTDNQTK VFAVITKKKE EKPLDYKYRY FRRVPVQEAD QSFHVGLQLC SSGHQRFNKL IWIHHSCHIT YKSTGETAVS AFEIDKMYTP LFFARVRSYT AFSERPL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FBXO9 (F-Box Protein 9 (FBXO9))

Alternative Name: FBXO9 (FBXO9 Products)

Synonyms: fbxo9 Protein, MGC75727 Protein, FBXO9 Protein, fbx9 Protein, FBX9 Protein, NY-REN-57 Protein, VCIA1 Protein, dJ341E18.2 Protein, wu:fc79g12 Protein, zgc:56212 Protein, zgc:77654 Protein, 9030401P18Rik Protein, AA986398 Protein, F-box protein 9 Protein, F-box only protein 9 Protein, f-box protein 9 Protein, F-box protein 9 L homeolog Protein, fbxo9 Protein, FBXO9 Protein, CpipJ_CPIJ012920 Protein, Tsp_03945 Protein, fbx9 Protein, Fbxo9 Protein, fbxo9.L Protein

Background: F-box only protein 9 (Cross-immune reaction antigen 1) (Renal carcinoma antigen NY-REN-57),FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins and plays a role in several biological processes such as cell cycle, cell proliferation, or maintenance of chromosome stability (PubMed:34480022, PubMed:23263282). Ubiquitinates mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is the activated due to the relief of feedback inhibition by mTORC1 (PubMed:23263282). Plays a role in maintaining epithelial cell survival by regulating the turn-over of chromatin modulator PRMT4 through ubiquitination and degradation by the proteasomal pathway (PubMed:34480022). Regulates also PPARgamma stability by facilitating PPARgamma/PPARG ubiquitination and thereby plays a role in adipocyte differentiation (By similarity). {ECO:0000250|UniProtKB:Q8BK06, ECO:0000269|PubMed:23263282, ECO:0000269|PubMed:34480022}.

Molecular Weight: 52.3 kDa

UniProt: Q9UK97