IL17RD Protein (AA 17-739) (rho-1D4 tag)

Catalog No. ABIN3092988

Product Details

Target: IL17RD (Interleukin 17 Receptor D (IL17RD))

Protein Type: Recombinant

Protein Characteristics: AA 17-739

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This IL17RD protein is labelled with rho-1D4 tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: CLNGSQLAVA AGGSGRARGA DTCGWRGVGP ASRNSGLYNI TFKYDNCTTY LNPVGKHVIA DAQNITISQY ACHDQVAVTI LWSPGALGIE FLKGFRVILE ELKSEGRQCQ QLILKDPKQL NSSFKRTGME SQPFLNMKFE TDYFVKVVPF PSIKNESNYH PFFFRTRACD LLLQPDNLAC KPFWKPRNLN ISQHGSDMQV SFDHAPHNFG FRFFYLHYKL KHEGPFKRKT CKQEQTTETT SCLLQNVSPG DYIIELVDDT NTTRKVMHYA LKPVHSPWAG PIRAVAITVP LVVISAFATL FTVMCRKKQQ ENIYSHLDEE SSESSTYTAA LPRERLRPRP KVFLCYSSKD GQNHMNVVQC FAYFLQDFCG CEVALDLWED FSLCREGQRE WVIQKIHESQ FIIVVCSKGM KYFVDKKNYK HKGGGRGSGK GELFLVAVSA IAEKLRQAKQ SSSAALSKFI AVYFDYSCEG DVPGILDLST KYRLMDNLPQ LCSHLHSRDH GLQEPGQHTR QGSRRNYFRS KSGRSLYVAI CNMHQFIDEE PDWFEKQFVP FHPPPLRYRE PVLEKFDSGL VLNDVMCKPG PESDFCLKVE AAVLGATGPA DSQHESQHGG LDQDGEARPA LDGSAALQPL LHTVKAGSPS DMPRDSGIYD SSVPSSELSL PLMEGLSTDQ TETSSLTESV SSSSGLGEEE PPALPSKLLS SGSCKADLGC RSYTDELHAV APL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human IL17RD Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IL17RD (Interleukin 17 Receptor D (IL17RD))

Alternative Name: IL17RD (IL17RD Products)

Synonyms: IL17RD Protein, sef Protein, il-17rd Protein, il17rlm Protein, HH18 Protein, IL-17RD Protein, IL17RLM Protein, SEF Protein, 2810004A10Rik Protein, AI428510 Protein, Sef Protein, Sef-S Protein, RGD1565237 Protein, cb208 Protein, id:ibd2875 Protein, id:ibd5059 Protein, zgc:136328 Protein, interleukin 17 receptor D Protein, IL17RD Protein, il17rd Protein, Il17rd Protein

Background: Feedback inhibitor of fibroblast growth factor mediated Ras-MAPK signaling and ERK activation. May inhibit FGF-induced FGFR1 tyrosine phosphorylation. Regulates the nuclear ERK signaling pathway by spatially blocking nuclear translocation of activated ERK without inhibiting cytoplasmic phosphorylation of ERK. Mediates JNK activation and may be involved in apoptosis. Might have a role in the early stages of fate specification of GnRH-secreting neurons (By similarity). {ECO:0000250, ECO:0000269|PubMed:12807873, ECO:0000269|PubMed:12958313, ECO:0000269|PubMed:15239952}.

Molecular Weight: 81.8 kDa Including tag.

UniProt: Q8NFM7

Pathways: Toll-Like Receptors Cascades