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IL22 Receptor alpha 1 Protein (AA 16-574) (rho-1D4 tag)

Crystallography grade IL22RA1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3092989
  • Target See all IL22 Receptor alpha 1 (IL22RA1) Proteins
    IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 16-574
    Origin
    • 8
    • 4
    • 3
    • 1
    • 1
    Human
    Source
    • 7
    • 5
    • 2
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This IL22 Receptor alpha 1 protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    HAPEDPSDLL QHVKFQSSNF ENILTWDSGP EGTPDTVYSI EYKTYGERDW VAKKGCQRIT RKSCNLTVET GNLTELYYAR VTAVSAGGRS ATKMTDRFSS LQHTTLKPPD VTCISKVRSI QMIVHPTPTP IRAGDGHRLT LEDIFHDLFY HLELQVNRTY QMHLGGKQRE YEFFGLTPDT EFLGTIMICV PTWAKESAPY MCRVKTLPDR TWTYSFSGAF LFSMGFLVAV LCYLSYRYVT KPPAPPNSLN VQRVLTFQPL RFIQEHVLIP VFDLSGPSSL AQPVQYSQIR VSGPREPAGA PQRHSLSEIT YLGQPDISIL QPSNVPPPQI LSPLSYAPNA APEVGPPSYA PQVTPEAQFP FYAPQAISKV QPSSYAPQAT PDSWPPSYGV CMEGSGKDSP TGTLSSPKHL RPKGQLQKEP PAGSCMLGGL SLQEVTSLAM EESQEAKSLH QPLGICTDRT SDPNVLHSGE EGTPQYLKGQ LPLLSSVQIE GHPMSLPLQP PSRPCSPSDQ GPSPWGLLES LVCPKDEAKS PAPETSDLEQ PTELDSLFRG LALTVQWES
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human IL22RA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product IL22RA1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    IL22 Receptor alpha 1 (IL22RA1) (Interleukin 22 Receptor, alpha 1 (IL22RA1))
    Alternative Name
    IL22RA1 (IL22RA1 Products)
    Synonyms
    IL22RA1 Protein, CRF2-9 Protein, IL22R Protein, IL22R1 Protein, 9130219A07Rik Protein, ENSMUSG00000073746 Protein, IL-22R Protein, Il22r Protein, RGD1559655 Protein, interleukin 22 receptor subunit alpha 1 Protein, interleukin 22 receptor, alpha 1 Protein, IL22RA1 Protein, Il22ra1 Protein
    Background
    Component of the receptor for IL20, IL22 and IL24. Component of IL22 receptor formed by IL22RA1 and IL10RB enabling IL22 signaling via JAK/STAT pathways. IL22 also induces activation of MAPK1/MAPK3 and Akt kinases pathways. Component of one of the receptor for IL20 and IL24 formed by IL22RA1 and IL20RB also signaling through STATs activation. Mediates IL24 antiangiogenic activity as well as IL24 inhibitory effect on endothelial cell tube formation and differentiation. {ECO:0000269|PubMed:11035029, ECO:0000269|PubMed:11564763, ECO:0000269|PubMed:11706020, ECO:0000269|PubMed:12351624, ECO:0000269|PubMed:12941841, ECO:0000269|PubMed:17204547}.
    Molecular Weight
    62.7 kDa Including tag.
    UniProt
    Q8N6P7
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