HYOU1 Protein (AA 33-999) (His tag)

Catalog No. ABIN3092996

Product Details

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Protein Type: Recombinant

Protein Characteristics: AA 33-999

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This HYOU1 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: LAVMSVDLGS ESMKVAIVKP GVPMEIVLNK ESRRKTPVIV TLKENERFFG DSAASMAIKN PKATLRYFQH LLGKQADNPH VALYQARFPE HELTFDPQRQ TVHFQISSQL QFSPEEVLGM VLNYSRSLAE DFAEQPIKDA VITVPVFFNQ AERRAVLQAA RMAGLKVLQL INDNTATALS YGVFRRKDIN TTAQNIMFYD MGSGSTVCTI VTYQMVKTKE AGMQPQLQIR GVGFDRTLGG LEMELRLRER LAGLFNEQRK GQRAKDVREN PRAMAKLLRE ANRLKTVLSA NADHMAQIEG LMDDVDFKAK VTRVEFEELC ADLFERVPGP VQQALQSAEM SLDEIEQVIL VGGATRVPRV QEVLLKAVGK EELGKNINAD EAAAMGAVYQ AAALSKAFKV KPFVVRDAVV YPILVEFTRE VEEEPGIHSL KHNKRVLFSR MGPYPQRKVI TFNRYSHDFN FHINYGDLGF LGPEDLRVFG SQNLTTVKLK GVGDSFKKYP DYESKGIKAH FNLDESGVLS LDRVESVFET LVEDSAEEES TLTKLGNTIS SLFGGGTTPD AKENGTDTVQ EEEESPAEGS KDEPGEQVEL KEEAEAPVED GSQPPPPEPK GDATPEGEKA TEKENGDKSE AQKPSEKAEA GPEGVAPAPE GEKKQKPARK RRMVEEIGVE LVVLDLPDLP EDKLAQSVQK LQDLTLRDLE KQEREKAANS LEAFIFETQD KLYQPEYQEV STEEQREEIS GKLSAASTWL EDEGVGATTV MLKEKLAELR KLCQGLFFRV EERKKWPERL SALDNLLNHS SMFLKGARLI PEMDQIFTEV EMTTLEKVIN ETWAWKNATL AEQAKLPATE KPVLLSKDIE AKMMALDREV QYLLNKAKFT KPRPRPKDKN GTRAEPPLNA SASDQGEKVI PPAGQTEDAE PISEPEKVET GSEPGDTEPL ELGGPGAEPE QKEQSTGQKR PLKNDEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HYOU1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: HYOU1 (HYOU1 Products)

Synonyms: GRP-170 Protein, Grp170 Protein, HSP12A Protein, ORP-150 Protein, ORP150 Protein, AI415631 Protein, CBP-140 Protein, Cab140 Protein, Orp150 Protein, id:ibd5056 Protein, wu:fd14c05 Protein, zgc:56019 Protein, HYOU1 Protein, orp150 Protein, GRP170 Protein, hypoxia up-regulated 1 Protein, hypoxia up-regulated protein 1 Protein, hypoxia up-regulated 1 S homeolog Protein, HYOU1 Protein, Hyou1 Protein, hyou1 Protein, LOC100380749 Protein, hyou1.S Protein

Background: Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {ECO:0000269|PubMed:10037731}.

Molecular Weight: 108.6 kDa Including tag.

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome