INTS3 Protein (AA 1-1043) (Strep Tag)

Catalog No. ABIN3093131

Product Details

Target: INTS3 (Integrator Complex Subunit 3 (INTS3))

Protein Type: Recombinant

Protein Characteristics: AA 1-1043

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This INTS3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MELQKGKGAA AAAAASGAAG GGGGGAGAGA PGGGRLLLST SLDAKDELEE RLERCMSIVT SMTAGVSERE ANDALNAYVC KGLPQHEEIC LGLFTLILTE PAQAQKCYRD LALVSRDGMN IVLNKINQIL MEKYLKLQDT CRTQLVWLVR ELVKSGVLGA DGVCMTFMKQ IAGGGDVTAK NIWLAESVLD ILTEQREWVL KSSILIAMAV YTYLRLIVDH HGTAQLQALR QKEVDFCISL LRERFMECLM IGRDLVRLLQ NVARIPEFEL LWKDIIHNPQ ALSPQFTGIL QLLQSRTSRK FLACRLTPDM ETKLLFMTSR VRFGQQKRYQ DWFQRQYLST PDSQSLRCDL IRYICGVVHP SNEVLSSDIL PRWAIIGWLL TTCTSNVAAS NAKLALFYDW LFFSPDKDSI MNIEPAILVM HHSMKPHPAI TATLLDFMCR IIPNFYPPLE GHVRQGVFSS LNHIVEKRVL AHLAPLFDNP KLDKELRAML REKFPEFCSS PSPPVEVKIE EPVSMEMDNH MSDKDESCYD NAEAAFSDDE EDLNSKGKKR EFRFHPIKET VVEEPVDITP YLDQLDESLR DKVLQLQKGS DTEAQCEVMQ EIVDQVLEED FDSEQLSVLA SCLQELFKAH FRGEVLPEEI TEESLEESVG KPLYLIFRNL CQMQEDNSSF SLLLDLLSEL YQKQPKIGYH LLYYLRASKA AAGKMNLYES FAQATQLGDL HTCLMMDMKA CQEDDVRLLC HLTPSIYTEF PDETLRSGEL LNMIVAVIDS AQLQELVCHV MMGNLVMFRK DSVLNILIQS LDWETFEQYC AWQLFLAHNI PLETIIPILQ HLKYKEHPEA LSCLLLQLRR EKPSEEMVKM VLSRPCHPDD QFTTSILRHW CMKHDELLAE HIKSLLIKNN SLPRKRQSLR SSSSKLAQLT LEQILEHLDN LRLNLTNTKQ NFFSQTPILQ ALQHVQASCD EAHKMKFSDL FSLAEEYEDS STKPPKSRRK AALSSPRSRK NATQPPNAEE ESGSSSASEE EDTKPKPTKR KRKGSSAVGS DSD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: INTS3 (Integrator Complex Subunit 3 (INTS3))

Alternative Name: INTS3 (INTS3 Products)

Synonyms: INTS3 Protein, Ints3 Protein, NV18802 Protein, ACYPI009450 Protein, DL3335W Protein, FCAALL.260 Protein, embryo defective 2739 Protein, C1orf193 Protein, C1orf60 Protein, INT3 Protein, SOSS-A Protein, C77668 Protein, RGD1304593 Protein, si:dkey-27c15.2 Protein, integrator complex subunit 3 Protein, integrator complex subunit 3 L homeolog Protein, embryo defective 2739 Protein, INTS3 Protein, ints3.L Protein, CpipJ_CPIJ008133 Protein, ints3 Protein, Ints3 Protein, LOC100168776 Protein, emb2739 Protein

Background: Integrator complex subunit 3 (Int3) (SOSS complex subunit A) (Sensor of single-strand DNA complex subunit A) (SOSS-A) (Sensor of ssDNA subunit A),FUNCTION: Component of the Integrator (INT) complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes (Probable). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex (PubMed:23904267). {ECO:0000269|PubMed:23904267, ECO:0000305|PubMed:16239144}., FUNCTION: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. The SOSS complex is required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. In the SOSS complex, it is required for the assembly of the complex and for stabilization of the complex at DNA damage sites. {ECO:0000269|PubMed:19605351, ECO:0000269|PubMed:19683501}.

Molecular Weight: 118.1 kDa

UniProt: Q68E01