Integrin Alpha2b Protein (CD41) (AA 32-993) (His tag)

Catalog No. ABIN3093188

Product Details

Target: Integrin Alpha2b (CD41)

Protein Type: Recombinant

Protein Characteristics: AA 32-993

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Integrin Alpha2b protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: LNLDPVQLTF YAGPNGSQFG FSLDFHKDSH GRVAIVVGAP RTLGPSQEET GGVFLCPWRA EGGQCPSLLF DLRDETRNVG SQTLQTFKAR QGLGASVVSW SDVIVACAPW QHWNVLEKTE EAEKTPVGSC FLAQPESGRR AEYSPCRGNT LSRIYVENDF SWDKRYCEAG FSSVVTQAGE LVLGAPGGYY FLGLLAQAPV ADIFSSYRPG ILLWHVSSQS LSFDSSNPEY FDGYWGYSVA VGEFDGDLNT TEYVVGAPTW SWTLGAVEIL DSYYQRLHRL RGEQMASYFG HSVAVTDVNG DGRHDLLVGA PLYMESRADR KLAEVGRVYL FLQPRGPHAL GAPSLLLTGT QLYGRFGSAI APLGDLDRDG YNDIAVAAPY GGPSGRGQVL VFLGQSEGLR SRPSQVLDSP FPTGSAFGFS LRGAVDIDDN GYPDLIVGAY GANQVAVYRA QPVVKASVQL LVQDSLNPAV KSCVLPQTKT PVSCFNIQMC VGATGHNIPQ KLSLNAELQL DRQKPRQGRR VLLLGSQQAG TTLNLDLGGK HSPICHTTMA FLRDEADFRD KLSPIVLSLN VSLPPTEAGM APAVVLHGDT HVQEQTRIVL DCGEDDVCVP QLQLTASVTG SPLLVGADNV LELQMDAANE GEGAYEAELA VHLPQGAHYM RALSNVEGFE RLICNQKKEN ETRVVLCELG NPMKKNAQIG IAMLVSVGNL EEAGESVSFQ LQIRSKNSQN PNSKIVLLDV PVRAEAQVEL RGNSFPASLV VAAEEGEREQ NSLDSWGPKV EHTYELHNNG PGTVNGLHLS IHLPGQSQPS DLLYILDIQP QGGLQCFPQP PVNPLKVDWG LPIPSPSPIH PAHHKRDRRQ IFLPEPEQPS RLQDPVLVSC DSAPCTVVQC DLQEMARGQR AMVTVLAFLW LPSLYQRPLD QFVLQSHAWF NVSSLPYAVP PLSLPRGEAQ VWTQLLRALE ER
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ITGA2B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Integrin Alpha2b (CD41)

Alternative Name: ITGA2B (CD41 Products)

Synonyms: AI172977 Protein, CD41 Protein, CD41B Protein, GpIIb Protein, alphaIIb Protein, BDPLT16 Protein, BDPLT2 Protein, GP2B Protein, GPIIb Protein, GT Protein, GTA Protein, HPA3 Protein, integrin alpha 2b Protein, integrin subunit alpha 2b Protein, Itga2b Protein, ITGA2B Protein

Background: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

Molecular Weight: 105.7 kDa Including tag.

UniProt: P08514

Pathways: Integrin Complex