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CD103 Protein (AA 19-1124) (His tag)

Crystallography grade ITGAE Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3093194
  • Target See all CD103 (ITGAE) Proteins
    CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 19-1124
    Origin
    • 5
    • 5
    Human
    Source
    • 7
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CD103 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    FNVDVARPWL TPKGGAPFVL SSLLHQDPST NQTWLLVTSP RTKRTPGPLH RCSLVQDEIL CHPVEHVPIP KGRHRGVTVV RSHHGVLICI QVLVRRPHSL SSELTGTCSL LGPDLRPQAQ ANFFDLENLL DPDARVDTGD CYSNKEGGGE DDVNTARQRR ALEKEEEEDK EEEEDEEEEE AGTEIAIILD GSGSIDPPDF QRAKDFISNM MRNFYEKCFE CNFALVQYGG VIQTEFDLRD SQDVMASLAR VQNITQVGSV TKTASAMQHV LDSIFTSSHG SRRKASKVMV VLTDGGIFED PLNLTTVINS PKMQGVERFA IGVGEEFKSA RTARELNLIA SDPDETHAFK VTNYMALDGL LSKLRYNIIS MEGTVGDALH YQLAQIGFSA QILDERQVLL GAVGAFDWSG GALLYDTRSR RGRFLNQTAA AAADAEAAQY SYLGYAVAVL HKTCSLSYIA GAPRYKHHGA VFELQKEGRE ASFLPVLEGE QMGSYFGSEL CPVDIDMDGS TDFLLVAAPF YHVHGEEGRV YVYRLSEQDG SFSLARILSG HPGFTNARFG FAMAAMGDLS QDKLTDVAIG APLEGFGADD GASFGSVYIY NGHWDGLSAS PSQRIRASTV APGLQYFGMS MAGGFDISGD GLADITVGTL GQAVVFRSRP VVRLKVSMAF TPSALPIGFN GVVNVRLCFE ISSVTTASES GLREALLNFT LDVDVGKQRR RLQCSDVRSC LGCLREWSSG SQLCEDLLLM PTEGELCEED CFSNASVKVS YQLQTPEGQT DHPQPILDRY TEPFAIFQLP YEKACKNKLF CVAELQLATT VSQQELVVGL TKELTLNINL TNSGEDSYMT SMALNYPRNL QLKRMQKPPS PNIQCDDPQP VASVLIMNCR IGHPVLKRSS AHVSVVWQLE ENAFPNRTAD ITVTVTNSNE RRSLANETHT LQFRHGFVAV LSKPSIMYVN TGQGLSHHKE FLFHVHGENL FGAEYQLQIC VPTKLRGLQV VAVKKLTRTQ ASTVCTWSQE RACAYSSVQH VEEWHSVSCV IASDKENVTV AAEISWDHSE ELLKDVTELQ ILGEISFNKS LYEGLNAENH RTKITVVFLK DEKYHS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ITGAE Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ITGAE Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))
    Alternative Name
    ITGAE (ITGAE Products)
    Synonyms
    A530055J10 Protein, CD103 Protein, aM290 Protein, alpha-E1 Protein, alpha-M290 Protein, HUMINAE Protein, integrin alpha E, epithelial-associated Protein, integrin subunit alpha E Protein, Itgae Protein, ITGAE Protein
    Background
    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
    Molecular Weight
    122.9 kDa Including tag.
    UniProt
    P38570
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