KIF11 Protein (AA 1-1056) (Strep Tag)

Catalog No. ABIN3093377

Product Details

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Protein Type: Recombinant

Protein Characteristics: AA 1-1056

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This KIF11 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MASQPNSSAK KKEEKGKNIQ VVVRCRPFNL AERKASAHSI VECDPVRKEV SVRTGGLADK SSRKTYTFDM VFGASTKQID VYRSVVCPIL DEVIMGYNCT IFAYGQTGTG KTFTMEGERS PNEEYTWEED PLAGIIPRTL HQIFEKLTDN GTEFSVKVSL LEIYNEELFD LLNPSSDVSE RLQMFDDPRN KRGVIIKGLE EITVHNKDEV YQILEKGAAK RTTAATLMNA YSSRSHSVFS VTIHMKETTI DGEELVKIGK LNLVDLAGSE NIGRSGAVDK RAREAGNINQ SLLTLGRVIT ALVERTPHVP YRESKLTRIL QDSLGGRTRT SIIATISPAS LNLEETLSTL EYAHRAKNIL NKPEVNQKLT KKALIKEYTE EIERLKRDLA AAREKNGVYI SEENFRVMSG KLTVQEEQIV ELIEKIGAVE EELNRVTELF MDNKNELDQC KSDLQNKTQE LETTQKHLQE TKLQLVKEEY ITSALESTEE KLHDAASKLL NTVEETTKDV SGLHSKLDRK KAVDQHNAEA QDIFGKNLNS LFNNMEELIK DGSSKQKAML EVHKTLFGNL LSSSVSALDT ITTVALGSLT SIPENVSTHV SQIFNMILKE QSLAAESKTV LQELINVLKT DLLSSLEMIL SPTVVSILKI NSQLKHIFKT SLTVADKIED QKKELDGFLS ILCNNLHELQ ENTICSLVES QKQCGNLTED LKTIKQTHSQ ELCKLMNLWT ERFCALEEKC ENIQKPLSSV QENIQQKSKD IVNKMTFHSQ KFCADSDGFS QELRNFNQEG TKLVEESVKH SDKLNGNLEK ISQETEQRCE SLNTRTVYFS EQWVSSLNER EQELHNLLEV VSQCCEASSS DITEKSDGRK AAHEKQHNIF LDQMTIDEDK LIAQNLELNE TIKIGLTKLN CFLEQDLKLD IPTGTTPQRK SYLYPSTLVR TEPREHLLDQ LKRKQPELLM MLNCSENNKE ETIPDVDVEE AVLGQYTEEP LSQEPSVDAG VDCSSIGGVP FFQHKKSHGK DKENRGINTL ERSKVEETTE HLVTKSRLPL RAQINL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Alternative Name: KIF11 (KIF11 Products)

Synonyms: EG5 Protein, HKSP Protein, KNSL1 Protein, MCLMR Protein, TRIP5 Protein, Eg5 Protein, Kif8 Protein, Kifl1 Protein, Knsl1 Protein, F2H17.19 Protein, F2H17_19 Protein, DDBDRAFT_0189194 Protein, DDBDRAFT_0201556 Protein, DDB_0189194 Protein, DDB_0201556 Protein, XLEg5K1 Protein, XlEg5 Protein, cos2 Protein, costal2 Protein, eg5 Protein, hksp Protein, kif11 Protein, kif11-a Protein, kif11-b Protein, kif11b Protein, knsl1 Protein, trip5 Protein, Cos2 Protein, Costal2 Protein, XLEg5K2 Protein, kif11a Protein, kinesin family member 11 Protein, P-loop containing nucleoside triphosphate hydrolases superfamily protein Protein, kinesin family member 11 S homeolog Protein, kinesin family member 11 L homeolog Protein, KIF11 Protein, Kif11 Protein, AT2G36200 Protein, kif11 Protein, kif11.S Protein, kif11.L Protein

Background: Kinesin-like protein KIF11 (Kinesin-like protein 1) (Kinesin-like spindle protein HKSP) (Kinesin-related motor protein Eg5) (Thyroid receptor-interacting protein 5) (TR-interacting protein 5) (TRIP-5),FUNCTION: Motor protein required for establishing a bipolar spindle and thus contributing to chromosome congression during mitosis (PubMed:19001501, PubMed:37728657). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769). {ECO:0000269|PubMed:19001501, ECO:0000269|PubMed:23857769}.

Molecular Weight: 119.2 kDa

UniProt: P52732