SLC43A1 Protein (AA 1-559) (Strep Tag)

Catalog No. ABIN3093483

Product Details

Target: SLC43A1 (Solute Carrier Family 43, Member 1 (SLC43A1))

Protein Type: Recombinant

Protein Characteristics: AA 1-559

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC43A1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAPTLQQAYR RRWWMACTAV LENLFFSAVL LGWGSLLIIL KNEGFYSSTC PAESSTNTTQ DEQRRWPGCD QQDEMLNLGF TIGSFVLSAT TLPLGILMDR FGPRPVRLVG SACFTASCTL MALASRDVEA LSPLIFLALS LNGFGGICLT FTSLTLPNMF GNLRSTLMAL MIGSYASSAI TFPGIKLIYD AGVAFVVIMF TWSGLACLIF LNCTLNWPIE AFPAPEEVNY TKKIKLSGLA LDHKVTGDLF YTHVTTMGQR LSQKAPSLED GSDAFMSPQD VRGTSENLPE RSVPLRKSLC SPTFLWSLLT MGMTQLRIIF YMAAVNKMLE YLVTGGQEHE TNEQQQKVAE TVGFYSSVFG AMQLLCLLTC PLIGYIMDWR IKDCVDAPTQ GTVLGDARDG VATKSIRPRY CKIQKLTNAI SAFTLTNLLL VGFGITCLIN NLHLQFVTFV LHTIVRGFFH SACGSLYAAV FPSNHFGTLT GLQSLISAVF ALLQQPLFMA MVGPLKGEPF WVNLGLLLFS LLGFLLPSYL FYYRARLQQE YAANGMGPLK VLSGSEVTA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC43A1 (Solute Carrier Family 43, Member 1 (SLC43A1))

Alternative Name: SLC43A1 (SLC43A1 Products)

Synonyms: 2610016F07Rik Protein, AA986141 Protein, Lat3 Protein, PB39 Protein, Pov1 Protein, R00504 Protein, LAT3 Protein, POV1 Protein, fi47a12 Protein, lat3a Protein, slc43a1 Protein, wu:fi47a12 Protein, zgc:55850 Protein, wu:fa01a01 Protein, zgc:158383 Protein, solute carrier family 43, member 1 Protein, solute carrier family 43 member 1 Protein, solute carrier family 43 (amino acid system L transporter), member 1a Protein, solute carrier family 43 (amino acid system L transporter), member 1b Protein, solute carrier family 43 member 1 L homeolog Protein, Slc43a1 Protein, SLC43A1 Protein, slc43a1a Protein, slc43a1b Protein, slc43a1.L Protein

Background: Large neutral amino acids transporter small subunit 3 (L-type amino acid transporter 3) (Prostate cancer overexpressed gene 1 protein) (Solute carrier family 43 member 1),FUNCTION: Uniport that mediates the transport of neutral amino acids such as L-leucine, L-isoleucine, L-valine, and L-phenylalanine (PubMed:12930836). The transport activity is sodium ions-independent, electroneutral and mediated by a facilitated diffusion (PubMed:12930836). {ECO:0000269|PubMed:12930836}.

Molecular Weight: 61.5 kDa

UniProt: O75387