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PPFIA4 Protein (AA 1-1185) (His tag)

Crystallography grade PPFIA4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3093573
  • Target See all PPFIA4 Proteins
    PPFIA4 (Protein tyrosine Phosphatase, Receptor Type, F Polypeptide (PTPRF), Interacting Protein (Liprin), alpha 4 (PPFIA4))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1185
    Origin
    Human
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This PPFIA4 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MCEVMPTINE GDRLGPPHGA DADANFEQLM VNMLDEREKL LESLRESQET LAATQSRLQD AIHERDQLQR HLNSALPQEF ATLTRELSMC REQLLEREEE ISELKAERNN TRLLLEHLEC LVSRHERSLR MTVVKRQAQS PSGVSSEVEV LKALKSLFEH HKALDEKVRE RLRAALERVT TLEEQLAGAH QQVSALQQGA GVRDGAAEEE GTVELGPKRL WKEDTGRVEE LQELLEKQNF ELSQARERLV TLTTTVTELE EDLGTARRDL IKSEELSSKH QRDLREALAQ KEDMEERITT LEKRYLAAQR EATSIHDLND KLENELANKE SLHRQCEEKA RHLQELLEVA EQKLQQTMRK AETLPEVEAE LAQRIAALTK AEERHGNIEE HLRQLEGQLE EKNQELARTA VQVRQREKMN EDHNKRLSDT VDRLLSESNE RLQLHLKERM AALEEKNTLI QELESSQRQI EEQHHHKGRL SEEIEKLRQE VDQLKGRGGP FVDGVHSRSH MGSAADVRFS LGTTTHAPPG VHRRYSALRE ESAKLALPLT VTLRSPTWMR MSQGVCCNLE YHSSGTLCGS SGPLPVPEMI QEEKESTELR AEEIETRVTS GSMEALNLKQ LRKRGSIPTS LTALSLASAS PPLSGRSTPK LTSRSAAQDL DRMGVMTLPS DLRKHRRKLL SPVSREENRE DKATIKCETS PPSSPRTLRL EKLGHPALSQ EEGKSALEDQ GSNPSSSNSS QDSLHKGAKR KGIKSSIGRL FGKKEKGRLI QLSRDGATGH VLLTDSEFSM QEPMVPAKLG TQAEKDRRLK KKHQLLEDAR RKGMPFAQWD GPTVVSWLEL WVGMPAWYVA ACRANVKSGA IMSALSDTEI QREIGISNAL HRLKLRLAIQ EMVSLTSPSA PPTSRTSSGN VWVTHEEMET LETSTKTDSE EGSWAQTLAY GDMNHEWIGN EWLPSLGLPQ YRSYFMECLV DARMLDHLTK KDLRVHLKMV DSFHRTSLQY GIMCLKRLNY DRKELEKRRE ESQHEIKDVL VWTNDQVVHW VQSIGLRDYA GNLHESGVHG ALLALDENFD HNTLALILQI PTQNTQARQV MEREFNNLLA LGTDRKLDDG DDKVFRRAPS WRKRFRPREH HGRGGMLSAS AETLPAGFRV STLGTLQPPP APPKKIMPEA HSHYLYGHML SAFRD
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PPFIA4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PPFIA4 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PPFIA4 (Protein tyrosine Phosphatase, Receptor Type, F Polypeptide (PTPRF), Interacting Protein (Liprin), alpha 4 (PPFIA4))
    Alternative Name
    PPFIA4 (PPFIA4 Products)
    Synonyms
    PTPRF interacting protein alpha 4 Protein, PPFIA4 Protein, Ppfia4 Protein
    Background
    May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates (By similarity). {ECO:0000250}.
    Molecular Weight
    135.4 kDa Including tag.
    UniProt
    O75335
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