LUZP1 Protein (AA 2-1076) (His tag)
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- Target See all LUZP1 Proteins
- LUZP1 (Leucine Zipper Protein 1 (LUZP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-1076
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This LUZP1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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AEFTSYKETA SSRHLRFKLQ SLSRRLDELE EATKNLQKAE DELLDLQDKV IQAEGSNSSM LAEIEVLRQR VLRIEGKDEE IKRAEDLCRL MKEKLEEEEN LTRELKSEIE RLQKRMAELE KLEEAFSRSK NDCTQLCLSL NEERNLTKKI SSELEMLRVK VKELESSEDR LDKTEQSLAS ELEKLKSLTL SFVSERKYLN EKEKENEKLI KELTQKLEQN KKMNRDYTRN ASNLERNDLR IEDGISSTLP SKESRRKGGL DYLKQVENET RNKSENEKNR NQEDNKVKDL NQEIEKLKTQ IKHFESLEEE LKKMKSKNND LQDNYLSEQN KNKLLASQLE EIKLQIKKQK ELENGEVEGE DAFLSSKGRH ERTKFRGHGS EASVSKHTAR ELSPQHKRER LRNREFALNN ENYSLSNRQV SSPSFTNRRA AKASHMGVST DSGTQETKKT EDRFVPGSSQ SEGKKSREQP SVLSRYPPAA QEHSKAWKGT SKPGTESGLK GKVEKTTRTF SDTTHGSVPS DPLGRADKAS DTSSETVFGK RGHVLGNGSQ VTQAANSGCS KAIGALASSR RSSSEGLSKG KKAANGLEAD NSCPNSKAPV LSKYPYSCRS QENILQGFST SHKEGVNQPA AVVMEDSSPH EALRCRVIKS SGREKPDSDD DLDIASLVTA KLVNTTITPE PEPKPQPNSR EKAKTRGAPR TSLFENDKDA GMENESVKSV RASTNTMELP DTNGAGVKSQ RPFSPREALR SRAIIKPVIV DKDVKKIMGG SGTETTLEKQ KPVSKPGPNK VTSSITIYPS DSSSPRAAPG EALRERHTST SNIQVGLAEL TSVSNHVSSP FELSIHKHDI TLQLAEAERM ADGPLKDRPE TVVSRSSIII KPSDPVERNS HAPPAETIRW KSHSAPSEVG FSDARHVTVR NAWKSRRDLK SLEDPPTRIG KNVESTNSNA YTQRSSTDFS ELEQPRSCLF EQGTRRVGPS SGDAPEPSSR RTQSSLTVSE VLTRRNRVGD TITVAAWNHS ASMEEEGEDC TLSVYRQLHN SLDPSELPGK QGLPESGRVR AEERLRPTRP CAEEN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human LUZP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product LUZP1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- LUZP1 (Leucine Zipper Protein 1 (LUZP1))
- Alternative Name
- LUZP1 (LUZP1 Products)
- Synonyms
- MGC80195 Protein, LUZP Protein, 2700072H04Rik Protein, AI266952 Protein, Luzp Protein, mFLJ00226 Protein, leucine zipper protein 1 Protein, leucine zipper protein 1 S homeolog Protein, LUZP1 Protein, luzp1.S Protein, luzp1 Protein, Luzp1 Protein
- Molecular Weight
- 121.1 kDa Including tag.
- UniProt
- Q86V48
- Pathways
- Tube Formation
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