MAGE-Like 2 Protein (MAGEL2) (AA 1-1249) (Strep Tag)

Catalog No. ABIN3093669

Product Details

Target: MAGE-Like 2 (MAGEL2)

Protein Type: Recombinant

Protein Characteristics: AA 1-1249

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This MAGE-Like 2 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MSQLSKNLGD SSPPAEAPKP PVYSRPTVLM RAPPASSRAP PVPWDPPPID LQASLAAWQA PQPAWEAPQG QLPAPVVPMT QPPALGGPIV PAPPLGGPMG KPPTPGVLMV HPPPPGAPMA QPPTPGVLMV HPSAPGAPMA HPPPPGTPMS HPPPPGTPMA HPPPPGTPMA HPPPPGTPMV HPPPPGTPMA HPPPPGTPMA HPPPPGTPMA HPPPPGTPMA HPPPPGTPMA QPPAPGVLMA QPLTPGVLMV QPAAPGAPMV QPPPAAMMTQ PQPSGAPMAK PPGPGVLMIH PPGARAPMTQ PPASGAPMAQ PAAPPAQPMA PPAQPMASWA PQAQPLILQI QSQVIRAPPQ VPQGPQAPPA QLATPPGWQA TSPGWQATQQ GWQATPLTWQ TTQVTWQAPA VTWQVPPPMR QGPPPIRPGP PPIRPGPPPV RQAPPLIRQA PPVIRQAPPV IRQAPPVIRQ APAVIRQAPP VIRQAPPVIR QAPPVIRQAP PLIRQAPPPI RPAPQVLATQ PPLWQALPPP PPLRQAPQAR LPAPQVQAAP QVPTAPPATQ VPAAPPAGPQ VPQPVLPAPL SAPLSAPQAV HCPSIIWQAP KGQPPVPHEI PTSMEFQEVQ QTQALAWQAQ KAPTHIWQPL PAQEAQRQAP PLVQLEQPFQ GAPPSQKAVQ IQLPPQQAQA SGPQAEVPTL PLQPSWQAPP AVLQAQPGPP VAAANFPLGS AKSLMTPSGE CRASSIDRRG SSKERRTSSK ERRAPSKDRM IFAATFCAPK AVSAARAHLP AAWKNLPATP ETFAPSSSVF PATSQFQPAS LNAFKGPSAA SETPKSLPYA LQDPFACVEA LPAVPWVPQP NMNASKASQA VPTFLMATAA APQATATTQE ASKTSVEPPR RSGKATRKKK HLEAQEDSRG HTLAFHDWQG PRPWENLNLS DWEVQSPIQV SGDWEHPNTP RGLSGWEGPS TSRILSGWEG PSASWALSAW EGPSTSRALG LSESPGSSLP VVVSEVASVS PGSSATQDNS KVEAQPLSPL DERANALVQF LLVKDQAKVP VQRSEMVKVI LREYKDECLD IINRANNKLE CAFGYQLKEI DTKNHAYIII NKLGYHTGNL VASYLDRPKF GLLMVVLSLI FMKGNCVRED LIFNFLFKLG LDVRETNGLF GNTKKLITEV FVRQKYLEYR RIPYTEPAEY EFLWGPRAFL ETSKMLVLRF LAKLHKKDPQ SWPFHYLEAL AECEWEDTDE DEPDTGDSAH GPTSRPPPR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: MAGE-Like 2 (MAGEL2)

Alternative Name: MAGEL2 (MAGEL2 Products)

Synonyms: NDNL1 Protein, nM15 Protein, MAGEL2 Protein, Mage-l2 Protein, ns7 Protein, MAGE family member L2 Protein, melanoma antigen, family L, 2 Protein, MAGEL2 Protein, Magel2 Protein

Background: MAGE-like protein 2 (Necdin-like protein 1) (Protein nM15),FUNCTION: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (PubMed:23452853). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (By similarity). {ECO:0000250|UniProtKB:Q9QZ04, ECO:0000269|PubMed:20864041, ECO:0000269|PubMed:23452853}.

Molecular Weight: 132.8 kDa

UniProt: Q9UJ55