NEO1 Protein (AA 34-1461) (rho-1D4 tag)

Catalog No. ABIN3094078

Product Details

Target: NEO1 (Neogenin 1 (NEO1))

Protein Type: Recombinant

Protein Characteristics: AA 34-1461

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This NEO1 protein is labelled with rho-1D4 tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: ARSGSAPQSP GASIRTFTPF YFLVEPVDTL SVRGSSVILN CSAYSEPSPK IEWKKDGTFL NLVSDDRRQL LPDGSLFISN VVHSKHNKPD EGYYQCVATV ESLGTIISRT AKLIVAGLPR FTSQPEPSSV YAGNNAILNC EVNADLVPFV RWEQNRQPLL LDDRVIKLPS GMLVISNATE GDGGLYRCVV ESGGPPKYSD EVELKVLPDP EVISDLVFLK QPSPLVRVIG QDVVLPCVAS GLPTPTIKWM KNEEALDTES SERLVLLAGG SLEISDVTED DAGTYFCIAD NGNETIEAQA ELTVQAQPEF LKQPTNIYAH ESMDIVFECE VTGKPTPTVK WVKNGDMVIP SDYFKIVKEH NLQVLGLVKS DEGFYQCIAE NDVGNAQAGA QLIILEHAPA TTGPLPSAPR DVVASLVSTR FIKLTWRTPA SDPHGDNLTY SVFYTKEGIA RERVENTSHP GEMQVTIQNL MPATVYIFRV MAQNKHGSGE SSAPLRVETQ PEVQLPGPAP NLRAYAASPT SITVTWETPV SGNGEIQNYK LYYMEKGTDK EQDVDVSSHS YTINGLKKYT EYSFRVVAYN KHGPGVSTPD VAVRTLSDVP SAAPQNLSLE VRNSKSIMIH WQPPAPATQN GQITGYKIRY RKASRKSDVT ETLVSGTQLS QLIEGLDRGT EYNFRVAALT INGTGPATDW LSAETFESDL DETRVPEVPS SLHVRPLVTS IVVSWTPPEN QNIVVRGYAI GYGIGSPHAQ TIKVDYKQRY YTIENLDPSS HYVITLKAFN NVGEGIPLYE SAVTRPHTDT SEVDLFVINA PYTPVPDPTP MMPPVGVQAS ILSHDTIRIT WADNSLPKHQ KITDSRYYTV RWKTNIPANT KYKNANATTL SYLVTGLKPN TLYEFSVMVT KGRRSSTWSM TAHGTTFELV PTSPPKDVTV VSKEGKPKTI IVNWQPPSEA NGKITGYIIY YSTDVNAEIH DWVIEPVVGN RLTHQIQELT LDTPYYFKIQ ARNSKGMGPM SEAVQFRTPK ADSSDKMPND QASGSGGKGS RLPDLGSDYK PPMSGSNSPH GSPTSPLDSN MLLVIIVSVG VITIVVVVII AVFCTRRTTS HQKKKRAACK SVNGSHKYKG NSKDVKPPDL WIHHERLELK PIDKSPDPNP IMTDTPIPRN SQDITPVDNS MDSNIHQRRN SYRGHESEDS MSTLAGRRGM RPKMMMPFDS QPPQPVISAH PIHSLDNPHH HFHSSSLASP ARSHLYHPGS PWPIGTSMSL SDRANSTESV RNTPSTDTMP ASSSQTCCTD HQDPEGATSS SYLASSQEED SGQSLPTAHV RPSHPLKSFA VPAIPPPGPP TYDPALPSTP LLSQQALNHH IHSVKTASIG TLGRSRPPMP VVVPSAPEVQ ETTRMLEDSE SSYEPDELTK EMAHLEGLMK DLNAITTA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NEO1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NEO1 (Neogenin 1 (NEO1))

Alternative Name: NEO1 (NEO1 Products)

Synonyms: cb333 Protein, zneo1 Protein, zgc:109795 Protein, 2610028H22Rik Protein, AI327052 Protein, D930014N22Rik Protein, Igdcc2 Protein, IGDCC2 Protein, NGN Protein, neogenin 1a Protein, neogenin 1 Protein, neogenin Protein, neo1a Protein, NEO1 Protein, LOC100448839 Protein, Neo1 Protein

Background: Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response. {ECO:0000269|PubMed:21149453}.

Molecular Weight: 157.6 kDa Including tag.

UniProt: Q92859

Pathways: Transition Metal Ion Homeostasis, Regulation of Muscle Cell Differentiation, Tube Formation