PA2G4 Protein (AA 2-394) (His tag)

Catalog No. ABIN3094327

Product Details

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Protein Type: Recombinant

Protein Characteristics: AA 2-394

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This PA2G4 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: SGEDEQQEQT IAEDLVVTKY KMGGDIANRV LRSLVEASSS GVSVLSLCEK GDAMIMEETG KIFKKEKEMK KGIAFPTSIS VNNCVCHFSP LKSDQDYILK EGDLVKIDLG VHVDGFIANV AHTFVVDVAQ GTQVTGRKAD VIKAAHLCAE AALRLVKPGN QNTQVTEAWN KVAHSFNCTP IEGMLSHQLK QHVIDGEKTI IQNPTDQQKK DHEKAEFEVH EVYAVDVLVS SGEGKAKDAG QRTTIYKRDP SKQYGLKMKT SRAFFSEVER RFDAMPFTLR AFEDEKKARM GVVECAKHEL LQPFNVLYEK EGEFVAQFKF TVLLMPNGPM RITSGPFEPD LYKSEMEVQD AELKALLQSS ASRKTQKKKK KKASKTAENA TSGETLEENE AGD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PA2G4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Alternative Name: PA2G4 (PA2G4 Products)

Synonyms: EBP1 Protein, HG4-1 Protein, p38-2G4 Protein, Ebp1 Protein, pa2g4 Protein, si:dz150i12.2 Protein, wu:fb19b11 Protein, wu:ft56d05 Protein, zgc:86732 Protein, ebp1 Protein, hg4-1 Protein, p38-2g4 Protein, pa2g4-a Protein, PA2G4 Protein, DKFZp469G2132 Protein, Pa2g4 Protein, MGC81621 Protein, 38kDa Protein, AA672939 Protein, Plfap Protein, proliferation-associated 2G4 Protein, proliferation-associated 2G4, a Protein, proliferation-associated 2G4, 38kDa Protein, proliferation-associated 2G4 L homeolog Protein, proliferation-associated protein 2G4 Protein, proliferation-associated 2G4 S homeolog Protein, proliferation-associated protein 2G4 pseudogene Protein, PA2G4 Protein, Pa2g4 Protein, pa2g4a Protein, pa2g4.L Protein, pa2g4 Protein, LOC100519773 Protein, pa2g4.S Protein, LOC693641 Protein

Background: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) (By similarity). Regulates cell proliferation, differentiation, and survival. Isoform 1 suppresses apoptosis whereas isoform 2 promotes cell differentiation (By similarity). {ECO:0000250|UniProtKB:P50580, ECO:0000250|UniProtKB:Q6AYD3, ECO:0000269|PubMed:11268000, ECO:0000269|PubMed:12682367, ECO:0000269|PubMed:15064750, ECO:0000269|PubMed:15583694, ECO:0000269|PubMed:16832058}.

Molecular Weight: 44.6 kDa Including tag.

UniProt: Q9UQ80

Pathways: Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades