PAN3 Protein (AA 1-887) (Strep Tag)

Catalog No. ABIN3094439

Product Details

Target: PAN3 (PAN3 Poly(A) Specific Ribonuclease Subunit Homolog (PAN3))

Protein Type: Recombinant

Protein Characteristics: AA 1-887

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PAN3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MNSGGGLPPP SAAASPSSSS LAAAVAVVAP PGVGGVPGGA AVGVKLKYCR YYAKDKTCFY GEECQFLHED PAAGAAPGLG LHSNSVPLAL AGAPVAGFPP GAVAGGGAGP PPGPKKPDLG DPGTGAAAGG GGSSGGLDGP RLAIPGMDGG ALTDTSLTDS YFSTSFIGVN GFGSPVETKY PLMQRMTNSS SSPSLLNDSA KPYSAHDPLT SPASSLFNDF GALNISQRRK PRKYRLGMLE ERLVPMGSKA RKAKNPIGCL ADRCKSGVPI NMVWWNRVTE NNLQTPNPTA SEFIPKGGST SRLSNVSQSN MSAFSQVFSH PSMGSPATAG LAPGMSLSAG SSPLHSPKIT PHTSPAPRRR SHTPNPASYM VPSSASTSVN NPVSQTPSSG QVIQKETVGG TTYFYTDTTP APLTGMVFPN YHIYPPTAPH VAYMQPKANA PSFFMADELR QELINRHLIT MAQIDQADMP AVPTEVDSYH SLFPLEPLPP PNRIQKSSNF GYITSCYKAV NSKDDLPYCL RRIHGFRLVN TKCMVLVDMW KKIQHSNIVT LREVFTTKAF AEPSLVFAYD FHAGGETMMS RHFNDPNADA YFTKRKWGQH EGPLPRQHAG LLPESLIWAY IVQLSSALRT IHTAGLACRV MDPTKILITG KTRLRVNCVG VFDVLTFDNS QNNNPLALMA QYQQADLISL GKVVLALACN SLAGIQRENL QKAMELVTIN YSSDLKNLIL YLLTDQNRMR SVNDIMPMIG ARFYTQLDAA QMRNDVIEED LAKEVQNGRL FRLLAKLGTI NERPEFQKDP TWSETGDRYL LKLFRDHLFH QVTEAGAPWI DLSHIISCLN KLDAGVPEKI SLISRDEKSV LVVTYSDLKR CFENTFQELI AAANGQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PAN3 (PAN3 Poly(A) Specific Ribonuclease Subunit Homolog (PAN3))

Alternative Name: PAN3 (PAN3 Products)

Synonyms: 2700050F09Rik Protein, A430027N15Rik Protein, AU014670 Protein, pan3 Protein, zgc:171628 Protein, PAN3 poly(A) specific ribonuclease subunit Protein, PAN3 poly(A) specific ribonuclease subunit S homeolog Protein, PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) Protein, Pan3 Protein, PAN3 Protein, pan3.S Protein, pan3 Protein

Background: PAN2-PAN3 deadenylation complex subunit PAN3 (PAB1P-dependent poly(A)-specific ribonuclease) (Poly(A)-nuclease deadenylation complex subunit 3) (PAN deadenylation complex subunit 3),FUNCTION: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decapping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins. {ECO:0000255|HAMAP-Rule:MF_03181, ECO:0000269|PubMed:14583602, ECO:0000269|PubMed:23932717}., FUNCTION: [Isoform 1]: Decreases PAN2-mediated deadenylation, possibly by preventing progression into the second CCR4-NOT mediated stage of biphasic deadenylation. Has a significant effect on mRNA stability, generally stabilizing a subset of the transcriptome. Stabilizes mRNAs degraded by the AU-rich element (ARE)-mediated mRNA decay pathway but promotes degradation of mRNAs by the microRNA-mediated pathway (PubMed:28559491). Its activity influences mRNP remodeling, specifically reducing formation of a subset of P-bodies containing GW220, an isoform of TNRC6A (PubMed:28559491). {ECO:0000269|PubMed:28559491}., FUNCTION: [Isoform 3]: Enhances PAN2 deadenylase activity and has an extensive effect on mRNA stability, generally enhancing mRNA decay across the transcriptome by multiple pathways, including the AU-rich element (ARE)-mediated pathway, microRNA-mediated pathway and the nonsense-mediated pathway (NMD) (PubMed:28559491). Its activity is required for efficient P-body formation (PubMed:28559491). May be involved in regulating mRNAs of genes involved in cell cycle progression and cell proliferation (PubMed:28559491). {ECO:0000269|PubMed:28559491}.

Molecular Weight: 95.6 kDa

UniProt: Q58A45