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PI4KB Protein (AA 2-816) (His tag)

Crystallography grade PI4KB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3094633
  • Target See all PI4KB Proteins
    PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-816
    Origin
    • 6
    • 1
    Human
    Source
    • 2
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This PI4KB protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    GDTVVEPAPL KPTSEPTSGP PGNNGGSLLS VITEGVGELS VIDPEVAQKA CQEVLEKVKL LHGGVAVSSR GTPLELVNGD GVDSEIRCLD DPPAQIREEE DEMGAAVASG TAKGARRRRQ NNSAKQSWLL RLFESKLFDI SMAISYLYNS KEPGVQAYIG NRLFCFRNED VDFYLPQLLN MYIHMDEDVG DAIKPYIVHR CRQSINFSLQ CALLLGAYSS DMHISTQRHS RGTKLRKLIL SDELKPAHRK RELPSLSPAP DTGLSPSKRT HQRSKSDATA SISLSSNLKR TASNPKVENE DEELSSSTES IDNSFSSPVR LAPEREFIKS LMAIGKRLAT LPTKEQKTQR LISELSLLNH KLPARVWLPT AGFDHHVVRV PHTQAVVLNS KDKAPYLIYV EVLECENFDT TSVPARIPEN RIRSTRSVEN LPECGITHEQ RAGSFSTVPN YDNDDEAWSV DDIGELQVEL PEVHTNSCDN ISQFSVDSIT SQESKEPVFI AAGDIRRRLS EQLAHTPTAF KRDPEDPSAV ALKEPWQEKV RRIREGSPYG HLPNWRLLSV IVKCGDDLRQ ELLAFQVLKQ LQSIWEQERV PLWIKPYKIL VISADSGMIE PVVNAVSIHQ VKKQSQLSLL DYFLQEHGSY TTEAFLSAQR NFVQSCAGYC LVCYLLQVKD RHNGNILLDA EGHIIHIDFG FILSSSPRNL GFETSAFKLT TEFVDVMGGL DGDMFNYYKM LMLQGLIAAR KHMDKVVQIV EIMQQGSQLP CFHGSSTIRN LKERFHMSMT EEQLQLLVEQ MVDGSMRSIT TKLYDGFQYL TNGIM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PI4KB Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PI4KB Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))
    Alternative Name
    PI4KB (PI4KB Products)
    Synonyms
    PIK4CB Protein, NV15725 Protein, PI4K-beta Protein, PI4Kbeta Protein, pik4cb Protein, NPIK Protein, PI4K-BETA Protein, PI4K92 Protein, PI4KBETA Protein, PI4KIIIBETA Protein, Pik4cb Protein, zgc:152715 Protein, AA437822 Protein, ESTM41 Protein, phosphatidylinositol 4-kinase, catalytic, beta Protein, phosphatidylinositol 4-kinase beta Protein, phosphatidylinositol 4-kinase, catalytic, beta L homeolog Protein, PI4KB Protein, LOC100115953 Protein, pi4kb Protein, Pi4kb Protein, pi4kb.L Protein
    Background
    Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity). {ECO:0000250, ECO:0000269|PubMed:10559940, ECO:0000269|PubMed:12749687, ECO:0000269|PubMed:9405935}.
    Molecular Weight
    92.2 kDa Including tag.
    UniProt
    Q9UBF8
    Pathways
    Inositol Metabolic Process
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