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CD45 Protein (AA 24-1304) (rho-1D4 tag)

Crystallography grade PTPRC Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3094840
  • Target See all CD45 (PTPRC) Proteins
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 24-1304
    Origin
    • 20
    • 10
    • 3
    • 1
    Human
    Source
    • 12
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CD45 protein is labelled with rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    QSPTPSPTGL TTAKMPSVPL SSDPLPTHTT AFSPASTFER ENDFSETTTS LSPDNTSTQV SPDSLDNASA FNTTGVSSVQ TPHLPTHADS QTPSAGTDTQ TFSGSAANAK LNPTPGSNAI SDVPGERSTA STFPTDPVSP LTTTLSLAHH SSAALPARTS NTTITANTSD AYLNASETTT LSPSGSAVIS TTTIATTPSK PTCDEKYANI TVDYLYNKET KLFTAKLNVN ENVECGNNTC TNNEVHNLTE CKNASVSISH NSCTAPDKTL ILDVPPGVEK FQLHDCTQVE KADTTICLKW KNIETFTCDT QNITYRFQCG NMIFDNKEIK LENLEPEHEY KCDSEILYNN HKFTNASKII KTDFGSPGEP QIIFCRSEAA HQGVITWNPP QRSFHNFTLC YIKETEKDCL NLDKNLIKYD LQNLKPYTKY VLSLHAYIIA KVQRNGSAAM CHFTTKSAPP SQVWNMTVSM TSDNSMHVKC RPPRDRNGPH ERYHLEVEAG NTLVRNESHK NCDFRVKDLQ YSTDYTFKAY FHNGDYPGEP FILHHSTSYN SKALIAFLAF LIIVTSIALL VVLYKIYDLH KKRSCNLDEQ QELVERDDEK QLMNVEPIHA DILLETYKRK IADEGRLFLA EFQSIPRVFS KFPIKEARKP FNQNKNRYVD ILPYDYNRVE LSEINGDAGS NYINASYIDG FKEPRKYIAA QGPRDETVDD FWRMIWEQKA TVIVMVTRCE EGNRNKCAEY WPSMEEGTRA FGDVVVKINQ HKRCPDYIIQ KLNIVNKKEK ATGREVTHIQ FTSWPDHGVP EDPHLLLKLR RRVNAFSNFF SGPIVVHCSA GVGRTGTYIG IDAMLEGLEA ENKVDVYGYV VKLRRQRCLM VQVEAQYILI HQALVEYNQF GETEVNLSEL HPYLHNMKKR DPPSEPSPLE AEFQRLPSYR SWRTQHIGNQ EENKSKNRNS NVIPYDYNRV PLKHELEMSK ESEHDSDESS DDDSDSEEPS KYINASFIMS YWKPEVMIAA QGPLKETIGD FWQMIFQRKV KVIVMLTELK HGDQEICAQY WGEGKQTYGD IEVDLKDTDK SSTYTLRVFE LRHSKRKDSR TVYQYQYTNW SVEQLPAEPK ELISMIQVVK QKLPQKNSSE GNKHHKSTPL LIHCRDGSQQ TGIFCALLNL LESAETEEVV DIFQVVKALR KARPGMVSTF EQYQFLYDVI ASTYPAQNGQ VKKNNHQEDK IEFDNEVDKV KQDANCVNPL GAPEKLPEAK EQAEGSEPTS GTEGPEHSVN GPASPALNQG S
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PTPRC Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PTPRC Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))
    Alternative Name
    PTPRC (PTPRC Products)
    Synonyms
    PTPRC Protein, si:dkeyp-47c5.1 Protein, B220 Protein, CD45 Protein, CD45R Protein, GP180 Protein, L-CA Protein, LCA Protein, LY5 Protein, T200 Protein, Cd45 Protein, Ly-5 Protein, Lyt-4 Protein, loc Protein, Lca Protein, RT7 Protein, cd45 Protein, protein tyrosine phosphatase, receptor type C Protein, protein tyrosine phosphatase, receptor type, C Protein, receptor-type tyrosine-protein phosphatase C Protein, PTPRC Protein, ptprc Protein, LOC100074271 Protein, Ptprc Protein
    Background
    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). {ECO:0000250, ECO:0000269|PubMed:11909961, ECO:0000269|PubMed:2845400}.
    Molecular Weight
    145.8 kDa Including tag.
    UniProt
    P08575
    Pathways
    TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling
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