RBMX Protein (AA 2-391) (His tag)

Catalog No. ABIN3094892

Product Details

Target: RBMX (RNA Binding Motif Protein, X-Linked (RBMX))

Protein Type: Recombinant

Protein Characteristics: AA 2-391

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This RBMX protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: VEADRPGKLF IGGLNTETNE KALEAVFGKY GRIVEVLLMK DRETNKSRGF AFVTFESPAD AKDAARDMNG KSLDGKAIKV EQATKPSFES GRRGPPPPPR SRGPPRGLRG GRGGSGGTRG PPSRGGHMDD GGYSMNFNMS SSRGPLPVKR GPPPRSGGPP PKRSAPSGPV RSSSGMGGRA PVSRGRDSYG GPPRREPLPS RRDVYLSPRD DGYSTKDSYS SRDYPSSRDT RDYAPPPRDY TYRDYGHSSS RDDYPSRGYS DRDGYGRDRD YSDHPSGGSY RDSYESYGNS RSAPPTRGPP PSYGGSSRYD DYSSSRDGYG GSRDSYSSSR SDLYSSGRDR VGRQERGLPP SMERGYPPPR DSYSSSSRGA PRGGGRGGSR SDRGGGRSRY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RBMX Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RBMX (RNA Binding Motif Protein, X-Linked (RBMX))

Alternative Name: RBMX (RBMX Products)

Synonyms: hnRNP G Protein, HNRNPG Protein, HNRPG Protein, RBMXP1 Protein, RBMXRT Protein, RNMX Protein, hnRNP-G Protein, fc66e11 Protein, wu:fb37c07 Protein, wu:fc66e11 Protein, hnrnpg Protein, hnrpg Protein, rbmxp1 Protein, rbmxrt Protein, rnmx Protein, RBMXL1 Protein, RNA binding motif protein, X chromosome Protein, RNA binding motif protein, X-linked Protein, RNA binding motif protein, X-linked S homeolog Protein, Rbmx Protein, RBMX Protein, rbmx Protein, rbmx.S Protein

Background: RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter, may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion, acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation, probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways, promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment. {ECO:0000269|PubMed:12165565, ECO:0000269|PubMed:12761049, ECO:0000269|PubMed:16707624, ECO:0000269|PubMed:18445477, ECO:0000269|PubMed:18541147, ECO:0000269|PubMed:19282290, ECO:0000269|PubMed:21327109}.

Molecular Weight: 43.2 kDa Including tag.

UniProt: P38159

Pathways: Chromatin Binding, Ribonucleoprotein Complex Subunit Organization