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RAB11A Protein (AA 2-213) (His tag)

Crystallography grade RAB11A Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Catalog No. ABIN3094910
  • Target See all RAB11A Proteins
    RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-213
    Origin
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 6
    • 4
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This RAB11A protein is labelled with His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Sequence
    GTRDDEYDYL FKVVLIGDSG VGKSNLLSRF TRNEFNLESK STIGVEFATR SIQVDGKTIK AQIWDTAGQE RYRAITSAYY RGAVGALLVY DIAKHLTYEN VERWLKELRD HADSNIVIML VGNKSDLRHL RAVPTDEARA FAEKNGLSFI ETSALDSTNV EAAFQTILTE IYRIVSQKQM SDRRENDMSP SNNVVPIHVP PTTENKPKVQ CC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RAB11A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product RAB11A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    RAB11A (RAB11A, Member RAS Oncogene Family (RAB11A))
    Alternative Name
    RAB11A (RAB11A Products)
    Synonyms
    rab11 Protein, RAB11A Protein, wu:fi15h09 Protein, zgc:103679 Protein, yl8 Protein, Rab11a Protein, rab11A Protein, DDBDRAFT_0190819 Protein, DDBDRAFT_0191190 Protein, DDB_0190819 Protein, DDB_0191190 Protein, YL8 Protein, RAB11 Protein, RAB11A, member RAS oncogene family S homeolog Protein, RAB11A, member RAS oncogene family Protein, RAB11a, member RAS oncogene family Protein, Rab11 GTPase Protein, rab11A protein Protein, Rab11a, GTPase Protein, Rab GTPase Protein, rab11A, RAB family GTPase Protein, rab11a.S Protein, RAB11A Protein, rab11a Protein, Rab11a Protein, rab11A Protein
    Background
    The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab regulates endocytic recycling. Acts as a major regulator of membrane delivery during cytokinesis. Together with MYO5B and RAB8A participates in epithelial cell polarization. Together with RAB3IP, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B participates in CFTR trafficking to the plasma membrane and TF (Transferrin) recycling in nonpolarized cells. Required in a complex with MYO5B and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Participates in the sorting and basolateral transport of CDH1 from the Golgi apparatus to the plasma membrane. Regulates the recycling of FCGRT (receptor of Fc region of monomeric Ig G) to basolateral membranes. May also play a role in melanosome transport and release from melanocytes. {ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:15689490, ECO:0000269|PubMed:17462998, ECO:0000269|PubMed:19542231, ECO:0000269|PubMed:20890297, ECO:0000269|PubMed:21282656}.
    Molecular Weight
    24.9 kDa Including tag.
    UniProt
    P62491
    Pathways
    Regulation of Cell Size, Thromboxane A2 Receptor Signaling, Regulation of long-term Neuronal Synaptic Plasticity
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