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Prosaposin Protein (PSAP) (AA 17-524) (His tag)

Crystallography grade PSAP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Catalog No. ABIN3095171
  • Target See all Prosaposin (PSAP) Proteins
    Prosaposin (PSAP)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 17-524
    Origin
    • 10
    • 6
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 11
    • 3
    • 2
    • 2
    • 2
    Insect Cells
    Purification tag / Conjugate
    This Prosaposin protein is labelled with His tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    GPVLGLKECT RGSAVWCQNV KTASDCGAVK HCLQTVWNKP TVKSLPCDIC KDVVTAAGDM LKDNATEEEI LVYLEKTCDW LPKPNMSASC KEIVDSYLPV ILDIIKGEMS RPGEVCSALN LCESLQKHLA ELNHQKQLES NKIPELDMTE VVAPFMANIP LLLYPQDGPR SKPQPKDNGD VCQDCIQMVT DIQTAVRTNS TFVQALVEHV KEECDRLGPG MADICKNYIS QYSEIAIQMM MHMQPKEICA LVGFCDEVKE MPMQTLVPAK VASKNVIPAL ELVEPIKKHE VPAKSDVYCE VCEFLVKEVT KLIDNNKTEK EILDAFDKMC SKLPKSLSEE CQEVVDTYGS SILSILLEEV SPELVCSMLH LCSGTRLPAL TVHVTQPKDG GFCEVCKKLV GYLDRNLEKN STKQEILAAL EKGCSFLPDP YQKQCDQFVA EYEPVLIEIL VEVMDPSFVC LKIGACPSAH KPLLGTEKCI WGPSYWCQNT ETAAQCNAVE HCKRHVWN
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human PSAP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product PSAP Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Prosaposin (PSAP)
    Alternative Name
    PSAP (PSAP Products)
    Synonyms
    GLBA Protein, SAP1 Protein, AI037048 Protein, SGP-1 Protein, SGP1A Protein, cb759 Protein, wu:fa14a06 Protein, wu:fb36e02 Protein, wu:fb58g07 Protein, PSAP Protein, PSP-A Protein, SFTPA1B Protein, SP-A Protein, prosaposin Protein, surfactant protein A1 Protein, PSAP Protein, Psap Protein, psap Protein, LOC100011417 Protein, LOC100304726 Protein, LOC100526719 Protein, SFTPA1 Protein
    Background
    Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate., Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases., Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12)., Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling. {ECO:0000250|UniProtKB:Q61207, ECO:0000269|PubMed:10383054}., Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
    Molecular Weight
    57.4 kDa Including tag.
    UniProt
    P07602
    Pathways
    Positive Regulation of Endopeptidase Activity
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