SLC26A6 Protein (AA 1-759) (Strep Tag)

Catalog No. ABIN3095187

Product Details

Target: SLC26A6 (Solute Carrier Family 26, Member 6 (SLC26A6))

Protein Type: Recombinant

Protein Characteristics: AA 1-759

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC26A6 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGLADASGPR DTQALLSATQ AMDLRRRDYH MERPLLNQEH LEELGRWGSA PRTHQWRTWL QCSRARAYAL LLQHLPVLVW LPRYPVRDWL LGDLLSGLSV AIMQLPQGLA YALLAGLPPV FGLYSSFYPV FIYFLFGTSR HISVGTFAVM SVMVGSVTES LAPQALNDSM INETARDAAR VQVASTLSVL VGLFQVGLGL IHFGFVVTYL SEPLVRGYTT AAAVQVFVSQ LKYVFGLHLS SHSGPLSLIY TVLEVCWKLP QSKVGTVVTA AVAGVVLVVV KLLNDKLQQQ LPMPIPGELL TLIGATGISY GMGLKHRFEV DVVGNIPAGL VPPVAPNTQL FSKLVGSAFT IAVVGFAIAI SLGKIFALRH GYRVDSNQEL VALGLSNLIG GIFQCFPVSC SMSRSLVQES TGGNSQVAGA ISSLFILLII VKLGELFHDL PKAVLAAIII VNLKGMLRQL SDMRSLWKAN RADLLIWLVT FTATILLNLD LGLVVAVIFS LLLVVVRTQM PHYSVLGQVP DTDIYRDVAE YSEAKEVRGV KVFRSSATVY FANAEFYSDA LKQRCGVDVD FLISQKKKLL KKQEQLKLKQ LQKEEKLRKQ AASPKGASVS INVNTSLEDM RSNNVEDCKM MQVSSGDKME DATANGQEDS KAPDGSTLKA LGLPQPDFHS LILDLGALSF VDTVCLKSLK NIFHDFREIE VEVYMAACHS PVVSQLEAGH FFDASITKKH LFASVHDAVT FALQHPRPVP DSPVSVTRL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC26A6 (Solute Carrier Family 26, Member 6 (SLC26A6))

Alternative Name: SLC26A6 (SLC26A6 Products)

Synonyms: B930010B04Rik Protein, CFEX Protein, Pat1 Protein, MGC81960 Protein, SLC26A6 Protein, slc26a6 Protein, MGC145603 Protein, si:dkey-20d21.8 Protein, solute carrier family 26 member 6 Protein, solute carrier family 26, member 6 Protein, solute carrier family 26 member 6 S homeolog Protein, anion exchanger SLC26A6 Protein, SLC26A6 Protein, Slc26a6 Protein, slc26a6.S Protein, slc26a6 Protein

Background: Solute carrier family 26 member 6 (Anion exchange transporter) (Pendrin-like protein 1) (Pendrin-L1),FUNCTION: Apical membrane anion-exchanger with wide epithelial distribution that plays a role as a component of the pH buffering system for maintaining acid-base homeostasis. Acts as a versatile DIDS-sensitive inorganic and organic anion transporter that mediates the uptake of monovalent anions like chloride, bicarbonate, formate and hydroxyl ion and divalent anions like sulfate and oxalate. Functions in multiple exchange modes involving pairs of these anions, which include chloride-bicarbonate, chloride-oxalate, oxalate-formate, oxalate-sulfate and chloride-formate exchange. Apical membrane chloride-bicarbonate exchanger that mediates luminal chloride absorption and bicarbonate secretion by the small intestinal brush border membrane and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption, possibly by providing a bicarbonate import pathway. Mediates also intestinal chloride absorption and oxalate secretion, thereby preventing hyperoxaluria and calcium oxalate urolithiasis. Transepithelial oxalate secretion, chloride-formate, chloride-oxalate and chloride-bicarbonate transport activities in the duodenum are inhibited by PKC activation in a calcium-independent manner. The apical membrane chloride-bicarbonate exchanger provides also a major route for fluid and bicarbonate secretion into the proximal tubules of the kidney as well as into the proximal part of the interlobular pancreatic ductal tree, where it mediates electrogenic chloride-bicarbonate exchange with a chloride-bicarbonate stoichiometry of 1:2, and hence will dilute and alkalinize protein-rich acinar secretion. Mediates also the transcellular sulfate absorption and oxalate secretion across the apical membrane in the duodenum and the formate ion efflux at the apical brush border of cells in the proximal tubules of kidney. Plays a role in sperm capacitation by increasing intracellular pH . {ECO:0000250|UniProtKB:Q8CIW6, ECO:0000269|PubMed:20501439, ECO:0000269|PubMed:27681177}., FUNCTION: [Isoform 4]: Apical membrane chloride-bicarbonate exchanger. Its association with carbonic anhydrase CA2 forms a bicarbonate transport metabolon, hence maximizes the local concentration of bicarbonate at the transporter site. {ECO:0000269|PubMed:15990874}.

Molecular Weight: 83.0 kDa

UniProt: Q9BXS9

Pathways: Dicarboxylic Acid Transport