SFI1 Protein (AA 1-1242) (Strep Tag)

Catalog No. ABIN3095321

Product Details

Target: SFI1 (Sfi1 Homolog, Spindle Assembly Associated (SFI1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1242

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SFI1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MKNLLTEKCI SSHNFHQKVI KQRMEKKVDS RYFKDGAVKK PYSAKTLSNK KSSASFGIRR ELPSTSHLVQ YRGTHTCTRQ GRLRELRIRC VARKFLYLWI RMTFGRVFPS KARFYYEQRL LRKVFEEWKE EWWVFQHEWK LCVRADCHYR YYLYNLMFQT WKTYVRQQQE MRNKYIRAEV HDAKQKMRQA WKSWLIYVVV RRTKLQMQTT ALEFRQRIIL RVWWSTWRQR LGQVRVSRAL HASALKHRAL SLQVQAWSQW REQLLYVQKE KQKVVSAVKH HQHWQKRRFL KAWLEYLQVR RVKRQQNEMA ERFHHVTVLQ IYFCDWQQAW ERRESLYAHH AQVEKLARKM ALRRAFTHWK HYMLLCAEEA AQFEMAEEHH RHSQLYFCFR ALKDNVTHAH LQQIRRNLAH QQHGVTLLHR FWNLWRSQIE QKKERELLPL LHAAWDHYRI ALLCKCIELW LQYTQKRRYK QLLQARADGH FQQRALPAAF HTWNRLWRWR HQENVLSARA TRFHRETLEK QVFSLWRQKM FQHRENRLAE RMAILHAERQ LLYRSWFMWH QQAAARHQEQ EWQTVACAHH RHGRLKKAFC LWRESAQGLR TERTGRVRAA EFHMAQLLRW AWSQWRECLA LRGAERQKLM RADLHHQHSV LHRALQAWVT YQGRVRSILR EVAARESQHN RQLLRGALRR WKENTMARVD EAKKTFQAST HYRRTICSKV LVQWREAVSV QMYYRQQEDC AIWEAQKVLD RGCLRTWFQR WWDCSRRSAQ QRLQLERAVQ HHHRQLLLEG LARWKTHHLQ CVRKRLLHRQ STQLLAQRLS RTCFRQWRQQ LAARRQEQRA TVRALWFWAF SLQAKVWATW LAFVLERRRK KARLQWALQA YQGQLLQEGA TRLLRFAASM KASRQQLQAQ QQVQAAHSLH RAVRRCATLW KQKVLGRGGK PQPLAAIAPS RKVTFEGPLL NRIAAGAGDG TLETKRPQAS RPLGALGRLA AEEPHALELN TAHSARKQPR RPHFLLEPAQ SQRPQKPQEH GLGMAQPAAP SLTRPFLAEA PTALVPHSPL PGALSSAPGP KQPPTASTGP ELLLLPLSSF MPCGAAAPAR VSAQRATPRD KPPVPSSLAS VPDPHLLLPG DFSATRAGPG LSTAGSLDLE AELEEIQQQL LHYQTTKQNL WSCRRQASSL RRWLELNREE PGPEDQEVEQ QVQKELEQVE MQIQLLAEEL QAQRQPIGAC VARIQALRQA LC
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SFI1 (Sfi1 Homolog, Spindle Assembly Associated (SFI1))

Alternative Name: SFI1 (SFI1 Products)

Synonyms: RGD1560636 Protein, PISD Protein, hSfi1p Protein, 2310047I15Rik Protein, C330046E03 Protein, mKIAA0542 Protein, SFI1 centrin binding protein Protein, Sfi1 homolog, spindle assembly associated (yeast) Protein, Sfi1 Protein, SFI1 Protein

Background: Protein SFI1 homolog (hSFI1),FUNCTION: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2. {ECO:0000269|PubMed:16956364}.

Molecular Weight: 147.7 kDa

UniProt: A8K8P3

Pathways: M Phase