SPPL2A Protein (AA 26-520) (rho-1D4 tag)

Catalog No. ABIN3095622

Product Details

Target: SPPL2A (Signal Peptide Peptidase-Like 2A (SPPL2A))

Protein Type: Recombinant

Protein Characteristics: AA 26-520

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This SPPL2A protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: QEAILHASGN GTTKDYCMLY NPYWTALPST LENATSISLM NLTSTPLCNL SDIPPVGIKS KAVVVPWGSC HFLEKARIAQ KGGAEAMLVV NNSVLFPPSG NRSEFPDVKI LIAFISYKDF RDMNQTLGDN ITVKMYSPSW PNFDYTMVVI FVIAVFTVAL GGYWSGLVEL ENLKAVTTED REMRKKKEEY LTFSPLTVVI FVVICCVMMV LLYFFYKWLV YVMIAIFCIA SAMSLYNCLA ALIHKIPYGQ CTIACRGKNM EVRLIFLSGL CIAVAVVWAV FRNEDRWAWI LQDILGIAFC LNLIKTLKLP NFKSCVILLG LLLLYDVFFV FITPFITKNG ESIMVELAAG PFGNNEKLPV VIRVPKLIYF SVMSVCLMPV SILGFGDIIV PGLLIAYCRR FDVQTGSSYI YYVSSTVAYA IGMILTFVVL VLMKKGQPAL LYLVPCTLIT ASVVAWRRKE MKKFWKGNSY QMMDHLDCAT NEENPVISGE QIVQQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human SPPL2A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SPPL2A (Signal Peptide Peptidase-Like 2A (SPPL2A))

Alternative Name: SPPL2A (SPPL2A Products)

Synonyms: RGD1563001 Protein, SPPL2A Protein, LOC453430 Protein, LOC100231183 Protein, IMP3 Protein, PSL2 Protein, 2010106G01Rik Protein, C130089K23Rik Protein, signal peptide peptidase-like 2A Protein, signal peptide peptidase like 2A Protein, Signal peptide peptidase-like 2A Protein, Sppl2a Protein, SPPL2A Protein, sppl2a Protein, psl2 Protein

Background: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing (PubMed:16829952, PubMed:16829951, PubMed:17557115, PubMed:17965014). Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus (PubMed:16829952). Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD) (PubMed:17557115). May play a role in the regulation of innate and adaptive immunity (PubMed:16829952). Catalyzes the intramembrane cleavage of the simian foamy virus envelope glycoprotein gp130 independently of prior ectodomain shedding by furin or furin-like proprotein convertase (PC)-mediated cleavage proteolysis (PubMed:23132852). {ECO:0000269|PubMed:16829951, ECO:0000269|PubMed:16829952, ECO:0000269|PubMed:17557115, ECO:0000269|PubMed:17965014, ECO:0000269|PubMed:23132852}.

Molecular Weight: 56.7 kDa Including tag.

UniProt: Q8TCT8