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SYNRG Protein (AA 1-1314) (His tag)

Crystallography grade SYNRG Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3095764
  • Target See all SYNRG Proteins
    SYNRG (Synergin gamma (SYNRG))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1314
    Origin
    • 3
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SYNRG protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MALRPGAGSG GGGAAGAGAG SAGGGGFMFP VAGGIRPPQA GLMPMQQQGF PMVSVMQPNM QGIMGMNYSS QMSQGPIAMQ AGIPMGPMPA AGMPYLGQAP FLGMRPPGPQ YTPDMQKQFA EEQQKRFEQQ QKLLEEERKR RQFEEQKQKL RLLSSVKPKT GEKSRDDALE AIKGNLDGFS RDAKMHPTPA SHPKKPGPSL EEKFLVSCDI STSGQEQIKL NTSEVGHKAL GPGSSKKYPS LMASNGVAVD GCVSGTTTAE AENTSDQNLS IEESGVGVFP SQDPAQPRMP PWIYNESLVP DAYKKILETT MTPTGIDTAK LYPILMSSGL PRETLGQIWA LANRTTPGKL TKEELYTVLA MIAVTQRGVP AMSPDALNQF PAAPIPTLSG FSMTLPTPVS QPTVIPSGPA GSMPLSLGQP VMGINLVGPV GGAAAQASSG FIPTYPANQV VKPEEDDFQD FQDASKSGSL DDSFSDFQEL PASSKTSNSQ HGNSAPSLLM PLPGTKALPS MDKYAVFKGI AADKSSENTV PPGDPGDKYS AFRELEQTAE NKPLGESFAE FRSAGTDDGF TDFKTADSVS PLEPPTKDKT FPPSFPSGTI QQKQQTQVKN PLNLADLDMF SSVNCSSEKP LSFSAVFSTS KSVSTPQSTG SAATMTALAA TKTSSLADDF GEFSLFGEYS GLAPVGEQDD FADFMAFSNS SISSEQKPDD KYDALKEEAS PVPLTSNVGS TVKGGQNSTA ASTKYDVFRQ LSLEGSGLGV EDLKDNTPSG KSDDDFADFH SSKFSSINSD KSLGEKAVAF RHTKEDSASV KSLDLPSIGG SSVGKEDSED ALSVQFDMKL ADVGGDLKHV MSDSSLDLPT VSGQHPPAAD IEDLKYAAFG SYSSNFAVST LTSYDWSDRD DATQGRKLSP FVLSAGSGSP SATSILQKKE TSFGSSENIT MTSLSKVTTF VSEDALPETT FPALASFKDT IPQTSEQKEY ENRDYKDFTK QDLPTAERSQ EATCPSPASS GASQETPNEC SDDFGEFQSE KPKISKFDFL VATSQSKMKS SEEMIKSELA TFDLSVQGSH KRSLSLGDKE ISRSSPSPAL EQPFRDRSNT LNEKPALPVI RDKYKDLTGE VEENERYAYE WQRCLGSALN VIKKANDTLN GISSSSVCTE VIQSAQGMEY LLGVVEVYRV TKRVELGIKA TAVCSEKLQQ LLKDIDKVWN NLIGFMSLAT LTPDENSLDF SSCMLRPGIK NAQELACGVC LLNVDSRSRK EEKPAEEHPK KAFNSETDSF KLAYGGHQYH ASCANFWINC VEPKPPGLVL PDLL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SYNRG Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SYNRG Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SYNRG (Synergin gamma (SYNRG))
    Alternative Name
    SYNRG (SYNRG Products)
    Synonyms
    AP1GBP1 Protein, MGC157114 Protein, Ap1gbp1 Protein, SYNG Protein, Syng Protein, synergin gamma Protein, synergin, gamma Protein, SYNRG Protein, Synrg Protein
    Background
    May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins.
    Molecular Weight
    141.6 kDa Including tag.
    UniProt
    Q9UMZ2
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