TCTN1 Protein (AA 23-587) (His tag)

Catalog No. ABIN3095814

Product Details

Target: TCTN1 (Tectonic Family Member 1 (TCTN1))

Protein Type: Recombinant

Protein Characteristics: AA 23-587

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This TCTN1 protein is labelled with His tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: QTDATPAVTT EGLNSTEAAL ATFGTFPSTR PPGTPRAPGP SSGPRPTPVT DVAVLCVCDL SPAQCDINCC CDPDCSSVDF SVFSACSVPV VTGDSQFCSQ KAVIYSLNFT ANPPQRVFEL VDQINPSIFC IHITNYKPAL SFINPEVPDE NNFDTLMKTS DGFTLNAESY VSFTTKLDIP TAAKYEYGVP LQTSDSFLRF PSSLTSSLCT DNNPAAFLVN QAVKCTRKIN LEQCEEIEAL SMAFYSSPEI LRVPDSRKKV PITVQSIVIQ SLNKTLTRRE DTDVLQPTLV NAGHFSLCVN VVLEVKYSLT YTDAGEVTKA DLSFVLGTVS SVVVPLQQKF EIHFLQENTQ PVPLSGNPGY VVGLPLAAGF QPHKGSGIIQ TTNRYGQLTI LHSTTEQDCL ALEGVRTPVL FGYTMQSGCK LRLTGALPCQ LVAQKVKSLL WGQGFPDYVA PFGNSQAQDM LDWVPIHFIT QSFNRKDSCQ LPGALVIEVK WTKYGSLLNP QAKIVNVTAN LISSSFPEAN SGNERTILIS TAVTFVDVSA PAEAGFRAPP AINARLPFNF FFPFV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TCTN1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TCTN1 (Tectonic Family Member 1 (TCTN1))

Alternative Name: TCTN1 (TCTN1 Products)

Synonyms: FLJ21127 Protein, JBTS13 Protein, TECT1 Protein, G730031O11Rik Protein, Tect1 Protein, RGD1566266 Protein, tectonic family member 1 Protein, tectonic family member 1 L homeolog Protein, TCTN1 Protein, tctn1.L Protein, tctn1 Protein, Tctn1 Protein

Background: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Regulator of Hedgehog (Hh), required for both activation and inhibition of the Hh pathway in the patterning of the neural tube. During neural tube development, it is required for formation of the most ventral cell types and for full Hh pathway activation. Functions in Hh signal transduction to fully activate the pathway in the presence of high Hh levels and to repress the pathway in the absence of Hh signals. Modulates Hh signal transduction downstream of SMO and RAB23 (By similarity). {ECO:0000250}.

Molecular Weight: 62.2 kDa Including tag.

UniProt: Q2MV58

Pathways: Tube Formation