TOP2B Protein (AA 2-1626) (His tag)

Catalog No. ABIN3095950

Product Details

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Protein Type: Recombinant

Protein Characteristics: AA 2-1626

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This TOP2B protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: AKSGGCGAGA GVGGGNGALT WVTLFDQNNA AKKEESETAN KNDSSKKLSV ERVYQKKTQL EHILLRPDTY IGSVEPLTQF MWVYDEDVGM NCREVTFVPG LYKIFDEILV NAADNKQRDK NMTCIKVSID PESNIISIWN NGKGIPVVEH KVEKVYVPAL IFGQLLTSSN YDDDEKKVTG GRNGYGAKLC NIFSTKFTVE TACKEYKHSF KQTWMNNMMK TSEAKIKHFD GEDYTCITFQ PDLSKFKMEK LDKDIVALMT RRAYDLAGSC RGVKVMFNGK KLPVNGFRSY VDLYVKDKLD ETGVALKVIH ELANERWDVC LTLSEKGFQQ ISFVNSIATT KGGRHVDYVV DQVVGKLIEV VKKKNKAGVS VKPFQVKNHI WVFINCLIEN PTFDSQTKEN MTLQPKSFGS KCQLSEKFFK AASNCGIVES ILNWVKFKAQ TQLNKKCSSV KYSKIKGIPK LDDANDAGGK HSLECTLILT EGDSAKSLAV SGLGVIGRDR YGVFPLRGKI LNVREASHKQ IMENAEINNI IKIVGLQYKK SYDDAESLKT LRYGKIMIMT DQDQDGSHIK GLLINFIHHN WPSLLKHGFL EEFITPIVKA SKNKQELSFY SIPEFDEWKK HIENQKAWKI KYYKGLGTST AKEAKEYFAD MERHRILFRY AGPEDDAAIT LAFSKKKIDD RKEWLTNFME DRRQRRLHGL PEQFLYGTAT KHLTYNDFIN KELILFSNSD NERSIPSLVD GFKPGQRKVL FTCFKRNDKR EVKVAQLAGS VAEMSAYHHG EQALMMTIVN LAQNFVGSNN INLLQPIGQF GTRLHGGKDA ASPRYIFTML STLARLLFPA VDDNLLKFLY DDNQRVEPEW YIPIIPMVLI NGAEGIGTGW ACKLPNYDAR EIVNNVRRML DGLDPHPMLP NYKNFKGTIQ ELGQNQYAVS GEIFVVDRNT VEITELPVRT WTQVYKEQVL EPMLNGTDKT PALISDYKEY HTDTTVKFVV KMTEEKLAQA EAAGLHKVFK LQTTLTCNSM VLFDHMGCLK KYETVQDILK EFFDLRLSYY GLRKEWLVGM LGAESTKLNN QARFILEKIQ GKITIENRSK KDLIQMLVQR GYESDPVKAW KEAQEKAAEE DETQNQHDDS SSDSGTPSGP DFNYILNMSL WSLTKEKVEE LIKQRDAKGR EVNDLKRKSP SDLWKEDLAA FVEELDKVES QEREDVLAGM SGKAIKGKVG KPKVKKLQLE ETMPSPYGRR IIPEITAMKA DASKKLLKKK KGDLDTAAVK VEFDEEFSGA PVEGAGEEAL TPSVPINKGP KPKREKKEPG TRVRKTPTSS GKPSAKKVKK RNPWSDDESK SESDLEETEP VVIPRDSLLR RAAAERPKYT FDFSEEEDDD ADDDDDDNND LEELKVKASP ITNDGEDEFV PSDGLDKDEY TFSPGKSKAT PEKSLHDKKS QDFGNLFSFP SYSQKSEDDS AKFDSNEEDS ASVFSPSFGL KQTDKVPSKT VAAKKGKPSS DTVPKPKRAP KQKKVVEAVN SDSDSEFGIP KKTTTPKGKG RGAKKRKASG SENEGDYNPG RKTSKTTSKK PKKTSFDQDS DVDIFPSDFP TEPPSLPRTG RARKEVKYFA ESDEEEDDVD FAMFN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TOP2B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: TOP2B (TOP2B Products)

Synonyms: TOP2B Protein, D230016L12Rik Protein, Top-2 Protein, TOPIIB Protein, top2beta Protein, DNA topoisomerase II beta Protein, topoisomerase (DNA) II beta Protein, microRNA 4442 Protein, TOP2B Protein, Top2b Protein, MIR4442 Protein

Background: Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. {ECO:0000269|PubMed:10684600}.

Molecular Weight: 184.1 kDa Including tag.

UniProt: Q02880