Tpp2 Protein (AA 2-1249) (His tag)

Catalog No. ABIN3096006

Product Details

Target: Tpp2 (Tripeptidyl Peptidase II (Tpp2))

Protein Type: Recombinant

Protein Characteristics: AA 2-1249

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Tpp2 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequence: ATAATEEPFP FHGLLPKKET GAASFLCRYP EYDGRGVLIA VLDTGVDPGA PGMQVTTDGK PKIVDIIDTT GSGDVNTATE VEPKDGEIVG LSGRVLKIPA SWTNPSGKYH IGIKNGYDFY PKALKERIQK ERKEKIWDPV HRVALAEACR KQEEFDVANN GSSQANKLIK EELQSQVELL NSFEKKYSDP GPVYDCLVWH DGEVWRACID SNEDGDLSKS TVLRNYKEAQ EYGSFGTAEM LNYSVNIYDD GNLLSIVTSG GAHGTHVASI AAGHFPEEPE RNGVAPGAQI LSIKIGDTRL STMETGTGLI RAMIEVINHK CDLVNYSYGE ATHWPNSGRI CEVINEAVWK HNIIYVSSAG NNGPCLSTVG CPGGTTSSVI GVGAYVSPDM MVAEYSLREK LPANQYTWSS RGPSADGALG VSISAPGGAI ASVPNWTLRG TQLMNGTSMS SPNACGGIAL ILSGLKANNI DYTVHSVRRA LENTAVKADN IEVFAQGHGI IQVDKAYDYL VQNTSFANKL GFTVTVGNNR GIYLRDPVQV AAPSDHGVGI EPVFPENTEN SEKISLQLHL ALTSNSSWVQ CPSHLELMNQ CRHINIRVDP RGLREGLHYT EVCGYDIASP NAGPLFRVPI TAVIAAKVNE SSHYDLAFTD VHFKPGQIRR HFIEVPEGAT WAEVTVCSCS SEVSAKFVLH AVQLVKQRAY RSHEFYKFCS LPEKGTLTEA FPVLGGKAIE FCIARWWASL SDVNIDYTIS FHGIVCTAPQ LNIHASEGIN RFDVQSSLKY EDLAPCITLK NWVQTLRPVS AKTKPLGSRD VLPNNRQLYE MVLTYNFHQP KSGEVTPSCP LLCELLYESE FDSQLWIIFD QNKRQMGSGD AYPHQYSLKL EKGDYTIRLQ IRHEQISDLE RLKDLPFIVS HRLSNTLSLD IHENHSFALL GKKKSSNLTL PPKYNQPFFV TSLPDDKIPK GAGPGCYLAG SLTLSKTELG KKADVIPVHY YLIPPPTKTK NGSKDKEKDS EKEKDLKEEF TEALRDLKIQ WMTKLDSSDI YNELKETYPN YLPLYVARLH QLDAEKERMK RLNEIVDAAN AVISHIDQTA LAVYIAMKTD PRPDAATIKN DMDKQKSTLV DALCRKGCAL ADHLLHTQAQ DGAISTDAEG KEEEGESPLD SLAETFWETT KWTDLFDNKV LTFAYKHALV NKMYGRGLKF ATKLVEEKPT KENWKNCIQL MKLLGWTHCA SFTENWLPIM YPPDYCVF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TPP2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Tpp2 (Tripeptidyl Peptidase II (Tpp2))

Alternative Name: TPP2 (Tpp2 Products)

Synonyms: TPP-2 Protein, TppII Protein, CG3991 Protein, Dmel\\CG3991 Protein, TPP II Protein, TPP2 Protein, TPPII Protein, anon-WO0118547.127 Protein, dTPP II Protein, dTPPII Protein, l(2)k14301 Protein, MGC83244 Protein, T13K14.10 Protein, T13K14_10 Protein, tripeptidyl peptidase ii Protein, DKFZp468E2313 Protein, tripeptidyl peptidase 2 Protein, tripeptidyl peptidase II Protein, tripeptidyl-peptidase II Protein, tripeptidyl peptidase 2 L homeolog Protein, tripeptidyl peptidase ii Protein, tripeptidyl-peptidase 2 Protein, TPP2 Protein, Tpp2 Protein, TppII Protein, tpp2.L Protein, LOC5572106 Protein, tpp2 Protein

Background: Component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin-proteasome pathway. May be able to complement the 26S proteasome function to some extent under conditions in which the latter is inhibited. Stimulates adipogenesis (By similarity). {ECO:0000250}.

Molecular Weight: 139.2 kDa Including tag.

UniProt: P29144