Triadin Protein (TRDN) (AA 69-729) (His tag)

Catalog No. ABIN3096021

Product Details

Target: Triadin (TRDN)

Protein Type: Recombinant

Protein Characteristics: AA 69-729

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Triadin protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: DLVDYKNFSA SSIAKIGSDP LKLVRDAMEE TTDWIYGFFS LLSDIISSED EEDDDGDEDT DKGEIDEPPL RKKEIHKDKT EKQEKPERKI QTKVTHKEKE KGKEKVREKE KPEKKATHKE KIEKKEKPET KTLAKEQKKA KTAEKSEEKT KKEVKGGKQE KVKQTAAKVK EVQKTPSKPK EKEDKEKAAV SKHEQKDQYA FCRYMIDIFV HGDLKPGQSP AIPPPLPTEQ ASRPTPASPA LEEKEGEKKK AEKKVTSETK KKEKEDIKKK SEKETAIDVE KKEPGKASET KQGTVKIAAQ AAAKKDEKKE DSKKTKKPAE VEQPKGKKQE KKEKHVEPAK SPKKEHSVPS DKQVKAKTER AKEEIGAVSI KKAVPGKKEE KTTKTVEQEI RKEKSGKTSS ILKDKEPIKG KEEKVPASLK EKEPETKKDE KMSKAGKEVK PKPPQLQGKK EEKPEPQIKK EAKPAISEKV QIHKQDIVKP EKTVSHGKPE EKVLKQVKAV TIEKTAKPKP TKKAEHRERE PPSIKTDKPK PTPKGTSEVT ESGKKKTEIS EKESKEKADM KHLREEKVST RKESLQLHNV TKAEKPARVS KDVEDVPASK KAKEGTEDVS PTKQKSPISF FQCVYLDGYN GYGFQFPFTP ADRPGESSGQ ANSPGQKQQG Q
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human TRDN Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Triadin (TRDN)

Alternative Name: TRDN (TRDN Products)

Synonyms: TRDN Protein, MGC140240 Protein, si:dkey-72g4.2 Protein, zgc:162523 Protein, DKFZp468F1917 Protein, trdn Protein, 2310045H21Rik Protein, EG432451 Protein, TDN Protein, TRISK Protein, triadin Protein, LOC100072982 Protein, EDI_200780 Protein, TRDN Protein, AOR_1_2668174 Protein, trdn Protein, Trdn Protein

Background: Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact (By similarity). Required for normal skeletal muscle strength. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats. {ECO:0000250|UniProtKB:E9Q9K5, ECO:0000269|PubMed:22422768}.

Molecular Weight: 75.4 kDa Including tag.

UniProt: Q13061

Pathways: Negative Regulation of Transporter Activity