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UGCGL2 Protein (AA 28-1516) (His tag)

Crystallography grade UGT2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3096173
  • Target See all UGCGL2 (UGT2) Proteins
    UGCGL2 (UGT2) (UDP-Glucose Glycoprotein Glucosyltransferase 2 (UGT2))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 28-1516
    Origin
    Human
    Source
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This UGCGL2 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    TVAASKSVTA HLAAKWPETP LLLEASEFMA EESNEKFWQF LETVQELAIY KQTESDYSYY NLILKKAGQF LDNLHINLLK FAFSIRAYSP AIQMFQQIAA DEPPPDGCNA FVVIHKKHTC KINEIKKLLK KAASRTRPYL FKGDHKFPTN KENLPVVILY AEMGTRTFSA FHKVLSEKAQ NEEILYVLRH YIQKPSSRKM YLSGYGVELA IKSTEYKALD DTQVKTVTNT TVEDETETNE VQGFLFGKLK EIYSDLRDNL TAFQKYLIES NKQMMPLKVW ELQDLSFQAA SQIMSAPVYD SIKLMKDISQ NFPIKARSLT RIAVNQHMRE EIKENQKDLQ VRFKIQPGDA RLFINGLRVD MDVYDAFSIL DMLKLEGKMM NGLRNLGING EDMSKFLKLN SHIWEYTYVL DIRHSSIMWI NDLENDDLYI TWPTSCQKLL KPVFPGSVPS IRRNFHNLVL FIDPAQEYTL DFIKLADVFY SHEVPLRIGF VFILNTDDEV DGANDAGVAL WRAFNYIAEE FDISEAFISI VHMYQKVKKD QNILTVDNVK SVLQNTFPHA NIWDILGIHS KYDEERKAGA SFYKMTGLGP LPQALYNGEP FKHEEMNIKE LKMAVLQRMM DASVYLQREV FLGTLNDRTN AIDFLMDRNN VVPRINTLIL RTNQQYLNLI STSVTADVED FSTFFFLDSQ DKSAVIAKNM YYLTQDDESI ISAVTLWIIA DFDKPSGRKL LFNALKHMKT SVHSRLGIIY NPTSKINEEN TAISRGILAA FLTQKNMFLR SFLGQLAKEE IATAIYSGDK IKTFLIEGMD KNAFEKKYNT VGVNIFRTHQ LFCQDVLKLR PGEMGIVSNG RFLGPLDEDF YAEDFYLLEK ITFSNLGEKI KGIVENMGIN ANNMSDFIMK VDALMSSVPK RASRYDVTFL RENHSVIKTN PQENDMFFNV IAIVDPLTRE AQKMAQLLVV LGKIINMKIK LFMNCRGRLS EAPLESFYRF VLEPELMSGA NDVSSLGPVA KFLDIPESPL LILNMITPEG WLVETVHSNC DLDNIHLKDT EKTVTAEYEL EYLLLEGQCF DKVTEQPPRG LQFTLGTKNK PAVVDTIVMA HHGYFQLKAN PGAWILRLHQ GKSEDIYQIV GHEGTDSQAD LEDIIVVLNS FKSKILKVKV KKETDKIKED ILTDEDEKTK GLWDSIKSFT VSLHKENKKE KDVLNIFSVA SGHLYERFLR IMMLSVLRNT KTPVKFWLLK NYLSPTFKEV IPHMAKEYGF RYELVQYRWP RWLRQQTERQ RIIWGYKILF LDVLFPLAVD KIIFVDADQI VRHDLKELRD FDLDGAPYGY TPFCDSRREM DGYRFWKTGY WASHLLRRKY HISALYVVDL KKFRRIGAGD RLRSQYQALS QDPNSLSNLD QDLPNNMIYQ VAIKSLPQDW LWCETWCDDE SKQRAKTIDL CNNPKTKESK LKAAARIVPE WVEYDAEIRQ LLDHLENKKQ DTILTHDEL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human UGGT2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product UGT2 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    UGCGL2 (UGT2) (UDP-Glucose Glycoprotein Glucosyltransferase 2 (UGT2))
    Alternative Name
    UGGT2 (UGT2 Products)
    Synonyms
    UGCGL2 Protein, ugcgl2 Protein, im:7146988 Protein, wu:fi13a08 Protein, HUGT2 Protein, UGT2 Protein, 1810064L21Rik Protein, 3110001A05Rik Protein, 3110027P15Rik Protein, A230065J02Rik Protein, AW047562 Protein, Ugcgl2 Protein, UDP-glucose glycoprotein glucosyltransferase 2 Protein, UGGT2 Protein, uggt2 Protein, Uggt2 Protein
    Background
    Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation (By similarity). {ECO:0000250}.
    Molecular Weight
    173.0 kDa Including tag.
    UniProt
    Q9NYU1
    Pathways
    SARS-CoV-2 Protein Interactome
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