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UNC5B Protein (AA 27-945) (rho-1D4 tag)

Crystallography grade UNC5B Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Catalog No. ABIN3096242
  • Target See all UNC5B Proteins
    UNC5B (Netrin receptor UNC5B (UNC5B))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 27-945
    Origin
    • 6
    • 2
    • 1
    • 1
    Human
    Source
    • 3
    • 3
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This UNC5B protein is labelled with rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequence
    GTDSGSEVLP DSFPSAPAEP LPYFLQEPQD AYIVKNKPVE LRCRAFPATQ IYFKCNGEWV SQNDHVTQEG LDEATGLRVR EVQIEVSRQQ VEELFGLEDY WCQCVAWSSA GTTKSRRAYV RIAYLRKNFD QEPLGKEVPL DHEVLLQCRP PEGVPVAEVE WLKNEDVIDP TQDTNFLLTI DHNLIIRQAR LSDTANYTCV AKNIVAKRRS TTATVIVYVN GGWSSWAEWS PCSNRCGRGW QKRTRTCTNP APLNGGAFCE GQAFQKTACT TICPVDGAWT EWSKWSACST ECAHWRSREC MAPPPQNGGR DCSGTLLDSK NCTDGLCMQN KKTLSDPNSH LLEASGDAAL YAGLVVAIFV VVAILMAVGV VVYRRNCRDF DTDITDSSAA LTGGFHPVNF KTARPSNPQL LHPSVPPDLT ASAGIYRGPV YALQDSTDKI PMTNSPLLDP LPSLKVKVYS SSTTGSGPGL ADGADLLGVL PPGTYPSDFA RDTHFLHLRS ASLGSQQLLG LPRDPGSSVS GTFGCLGGRL SIPGTGVSLL VPNGAIPQGK FYEMYLLINK AESTLPLSEG TQTVLSPSVT CGPTGLLLCR PVILTMPHCA EVSARDWIFQ LKTQAHQGHW EEVVTLDEET LNTPCYCQLE PRACHILLDQ LGTYVFTGES YSRSAVKRLQ LAVFAPALCT SLEYSLRVYC LEDTPVALKE VLELERTLGG YLVEEPKPLM FKDSYHNLRL SLHDLPHAHW RSKLLAKYQE IPFYHIWSGS QKALHCTFTL ERHSLASTEL TCKICVRQVE GEGQIFQLHT TLAETPAGSL DTLCSAPGST VTTQLGPYAF KIPLSIRQKI CNSLDAPNSR GNDWRMLAQK LSMDRYLNYF ATKASPTGVI LDLWEALQQD DGDLNSLASA LEEMGKSEML VAVATDGDC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human UNC5B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product UNC5B Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    UNC5B (Netrin receptor UNC5B (UNC5B))
    Alternative Name
    UNC5B (UNC5B Products)
    Synonyms
    unc5h2 Protein, 6330415E02Rik Protein, A630020F16 Protein, D10Bwg0792e Protein, Unc5h2 Protein, UNC5H2 Protein, p53RDL1 Protein, UNC5C Protein, UNC5B Protein, p53rdl1 Protein, unc-5 netrin receptor B Protein, unc-5 netrin receptor B L homeolog Protein, unc5b Protein, Unc5b Protein, UNC5B Protein, unc5b.L Protein
    Background
    Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion (By similarity). Functions as netrin receptor that negatively regulates vascular branching during angiogenesis. Mediates retraction of tip cell filopodia on endothelial growth cones in response to netrin (By similarity). It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand (PubMed:12598906). Mediates apoptosis by activating DAPK1. In the absence of NTN1, activates DAPK1 by reducing its autoinhibitory phosphorylation at Ser-308 thereby increasing its catalytic activity (By similarity). {ECO:0000250|UniProtKB:O08722, ECO:0000250|UniProtKB:Q8K1S3, ECO:0000269|PubMed:12598906}.
    Molecular Weight
    102.1 kDa Including tag.
    UniProt
    Q8IZJ1
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