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ZDHHC8 Protein (AA 1-765) (Strep Tag)

Crystallography grade ZDHHC8 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Catalog No. ABIN3096449
  • Target See all ZDHHC8 Proteins
    ZDHHC8 (Zinc Finger, DHHC-Type Containing 8 (ZDHHC8))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-765
    Origin
    Human
    Source
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This ZDHHC8 protein is labelled with Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Sequence
    MPRSPGTRLK PAKYIPVATA AALLVGSSTL FFVFTCPWLT RAVSPAVPVY NGIIFLFVLA NFSMATFMDP GVFPRADEDE DKEDDFRAPL YKNVDVRGIQ VRMKWCATCH FYRPPRCSHC SVCDNCVEDF DHHCPWVNNC IGRRNYRYFF LFLLSLSAHM VGVVAFGLVY VLNHAEGLGA AHTTITMAVM CVAGLFFIPV IGLTGFHVVL VTRGRTTNEQ VTGKFRGGVN PFTRGCCGNV EHVLCSPLAP RYVVEPPRLP LAVSLKPPFL RPELLDRAAP LKVKLSDNGL KAGLGRSKSK GSLDRLDEKP LDLGPPLPPK IEAGTFSSDL QTPRPGSAES ALSVQRTSPP TPAMYKFRPA FPTGPKVPFC GPGEQVPGPD SLTLGDDSIR SLDFVSEPSL DLPDYGPGGL HAAYPPSPPL SASDAFSGAL RSLSLKASSR RGGDHVALQP LRSEGGPPTP HRSIFAPHAL PNRNGSLSYD SLLNPGSPGG HACPAHPAVG VAGYHSPYLH PGATGDPPRP LPRSFSPVLG PRPREPSPVR YDNLSRTIMA SIQERKDREE RERLLRSQAD SLFGDSGVYD APSSYSLQQA SVLSEGPRGP ALRYGSRDDL VAGPGFGGAR NPALQTSLSS LSSSVSRAPR TSSSSLQADQ ASSNAPGPRP SSGSHRSPAR QGLPSPPGTP HSPSYAGPKA VAFIHTDLPE PPPSLTVQRD HPQLKTPPSK LNGQSPGLAR LGPATGPPGP SASPTRHTLV KKVSGVGGTT YEISV
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Endotoxin Level
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Grade
    Crystallography grade
    Top Product
    Discover our top product ZDHHC8 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ZDHHC8 (Zinc Finger, DHHC-Type Containing 8 (ZDHHC8))
    Alternative Name
    ZDHHC8 (ZDHHC8 Products)
    Synonyms
    znf378 Protein, zdhhcl1 Protein, zdhhc8 Protein, DHHC8 Protein, ZDHHCL1 Protein, ZNF378 Protein, D16H22S1738E Protein, DHHC-8 Protein, E330009O14Rik Protein, Op53c05 Protein, zdhhc5 Protein, zisp Protein, zinc finger DHHC-type containing 8 Protein, membrane-associated DHHC8 zinc finger protein Protein, zinc finger, DHHC-type containing 8 Protein, zinc finger DHHC-type containing 8 S homeolog Protein, zinc finger, DHHC domain containing 8 Protein, zinc finger, DHHC-type containing 8b Protein, zdhhc8 Protein, ZDHHC8 Protein, Zdhhc8 Protein, zdhhc8.S Protein, zdhhc8b Protein
    Background
    Palmitoyltransferase ZDHHC8 (EC 2.3.1.225) (Zinc finger DHHC domain-containing protein 8) (DHHC-8) (Zinc finger protein 378),FUNCTION: Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates and therefore functions in several unrelated biological processes (Probable). Through the palmitoylation of ABCA1 regulates the localization of the transporter to the plasma membrane and thereby regulates its function in cholesterol and phospholipid efflux (Probable). Could also pamitoylate the D(2) dopamine receptor DRD2 and regulate its stability and localization to the plasma membrane (Probable). Could also play a role in glutamatergic transmission (By similarity). {ECO:0000250|UniProtKB:Q5Y5T5, ECO:0000305|PubMed:19556522, ECO:0000305|PubMed:23034182, ECO:0000305|PubMed:26535572}., FUNCTION: (Microbial infection) Able to palmitoylate SARS coronavirus-2/SARS-CoV-2 spike protein following its synthesis in the endoplasmic reticulum (ER). In the infected cell, promotes spike biogenesis by protecting it from premature ER degradation, increases half-life and controls the lipid organization of its immediate membrane environment. Once the virus has formed, spike palmitoylation controls fusion with the target cell. {ECO:0000269|PubMed:34599882}.
    Molecular Weight
    81.4 kDa
    UniProt
    Q9ULC8
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