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TAF7 Protein (AA 1-341) (His tag)

Crystallography grade TAF7 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3119932
  • Target See all TAF7 Proteins
    TAF7 (TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-341
    Origin
    • 4
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 4
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This TAF7 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MSKNKDDAPH ELESQFILRL PPEYAATVRR AVQSGHVNLK DKLSIELHPD GRHGIVRVDR VPLAAKLVDL PCVTESLKTI DKKTFYKTAD ISQMLVATVD GDLYPPVEEA AATADPKANK KKDKDKEKKF VWNHGITLPL KNVRKRRFRK TAKKKYIESP DVEKEVKRLL STDAEAVSTR WEIIAEDETK ETENQGLDIS SPGMSGHRQG HDSLEHDELR EIFNDLSSSS EDEEDVNILD TEEDLERQLQ DKLNESDEQH QENEGTNQLV MGIQKQIDNM KGKLQETQDR AKRQEDLIMK VENLALKNRF QAVLDELKQK EDREKEQLSS LQEELESLLE K
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Taf7 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TAF7 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TAF7 (TAF7 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 55kDa (TAF7))
    Alternative Name
    Taf7 (TAF7 Products)
    Synonyms
    CG2670 Protein, Dmel\\CG2670 Protein, E Protein, TAF Protein, TAF55 Protein, TAF7 Protein, TAF[[II]] Protein, TAF[[II]]55 Protein, TFIID Protein, Taf55 Protein, anon-84Ec Protein, dTAF[[II]]55 Protein, AO090012000400 Protein, CHUNP6888 Protein, 55kDa Protein, AI607308 Protein, BB007175 Protein, TAFII55 Protein, Taf2f Protein, TAF2F Protein, taf2f Protein, TBP-associated factor 7 Protein, transcription initiation factor tfiid 55 kDa subunit Protein, transcription initiation factor TFIID subunit D6 Protein, transcription initiation factor TFIID subunit 7 Protein, TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TATA-box binding protein associated factor 7 Protein, TATA-box binding protein associated factor 7 L homeolog Protein, Taf7 Protein, CC1G_01188 Protein, PGTG_01179 Protein, AOR_1_682194 Protein, taf7 Protein, TAF7 Protein, taf7.L Protein
    Background
    Functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Present in both of the previously described TFIID species which either lack or contain TAFII30 (TFIID alpha and TFIID beta respectively) (By similarity). {ECO:0000250}.
    Molecular Weight
    40.1 kDa Including tag.
    UniProt
    Q9R1C0
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway
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