Corin, Serine Peptidase (CORIN) (AA 868-1113) protein (rho-1D4 tag)

Details for Product No. ABIN3120429, Supplier: Log in to see
Protein Name
  • CORIN
  • CG2105
  • Dmel\\CG2105
  • SP75
  • LOC559109
  • AV273130
  • Lrp4
  • ATC2
  • CRN
  • PEE5
  • TMPRSS10
  • corin, serine peptidase
  • CG2105 gene product from transcript CG2105-RC
  • novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN)
  • corin
  • CORIN
  • Corin
  • CH1073-76A20.1
  • LOC100340894
Protein Characteristics
AA 868-1113
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Origin
Mouse (Murine)
15
8
2
Source
Insect Cells
13
10
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This Corin protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence RILGGRTSRP GRWPWQCSLQ SEPSGHICGC VLIAKKWVLT VAHCFEGRED ADVWKVVFGI NNLDHPSGFM QTRFVKTILL HPRYSRAVVD YDISVVELSD DINETSYVRP VCLPSPEEYL EPDTYCYITG WGHMGNKMPF KLQEGEVRII PLEQCQSYFD MKTITNRMIC AGYESGTVDS CMGDSGGPLV CERPGGQWTL FGLTSWGSVC FSKVLGPGVY SNVSYFVGWI ERQIYIQTFL QKKSQG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Corin Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Background Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. {ECO:0000269|PubMed:11884416, ECO:0000269|PubMed:15637153, ECO:0000269|PubMed:20613715, ECO:0000269|PubMed:22418978, ECO:0000269|PubMed:22437503}.
Molecular Weight 28.9 kDa Including tag.
UniProt Q9Z319
Research Area Proteolysis / Ubiquitin, Wnt, Cardiovascular
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Corin, Serine Peptidase (CORIN) (AA 868-1113) protein (rho-1D4 tag) (ABIN3120429) „Crystallography Grade“ protein due to multi-step, protein-specific purification process