beta Actin Protein (AA 2-375) (His tag)

Catalog No. ABIN3120662

Product Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Protein Type: Recombinant

Protein Characteristics: AA 2-375

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This beta Actin protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: DDDIAALVVD NGSGMCKAGF AGDDAPRAVF PSIVGRPRHQ GVMVGMGQKD SYVGDEAQSK RGILTLKYPI EHGIVTNWDD MEKIWHHTFY NELRVAPEEH PVLLTEAPLN PKANREKMTQ IMFETFNTPA MYVAIQAVLS LYASGRTTGI VMDSGDGVTH TVPIYEGYAL PHAILRLDLA GRDLTDYLMK ILTERGYSFT TTAEREIVRD IKEKLCYVAL DFEQEMATAA SSSSLEKSYE LPDGQVITIG NERFRCPEAL FQPSFLGMES CGIHETTFNS IMKCDVDIRK DLYANTVLSG GTTMYPGIAD RMQKEITALA PSTMKIKIIA PPERKYSVWI GGSILASLST FQQMWISKQE YDESGPSIVH RKCF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Actb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: Actb (ACTB Products)

Synonyms: BRWS1 Protein, PS1TP5BP1 Protein, ACTA1 Protein, Actx Protein, E430023M04Rik Protein, beta-actin Protein, ps1tp5bp1 Protein, act-2 Protein, actb Protein, B-actin Protein, LOC100174883 Protein, LOC100304816 Protein, A Protein, ACT5C Protein, Ac5C Protein, Act Protein, Act-5C Protein, Act5 Protein, Act5c Protein, Actin Protein, Actin/BAP47 Protein, Actin5C Protein, BAP47 Protein, Bap47 Protein, CG4027 Protein, Dmel\\CG4027 Protein, M32055 Protein, T11 Protein, act Protein, act 5C Protein, act5C Protein, actin Protein, actin5C Protein, anon-EST:fe2D2 Protein, beta-actin/Bap47 Protein, cyt5C Protein, l(1)G0009 Protein, l(1)G0010 Protein, l(1)G0025 Protein, l(1)G0079 Protein, l(1)G0117 Protein, l(1)G0177 Protein, l(1)G0245 Protein, l(1)G0330 Protein, l(1)G0420 Protein, l(1)G0486 Protein, Bact Protein, ACT-5 Protein, ACTB Protein, B-ACTZF Protein, actba Protein, bact Protein, bactin1 Protein, bactzf Protein, wu:fd18f05 Protein, A26C1B Protein, POTE2alpha Protein, POTEACTIN Protein, Beta-actin Protein, actin beta Protein, actin, beta Protein, cytoplasmic actin OlCA1 Protein, beta-actin Protein, Actin 5C Protein, actin gamma 1 Protein, actin, beta 1 Protein, actin, beta L homeolog Protein, beta actin Protein, POTE ankyrin domain family member F Protein, actin, beta like 2 Protein, actin, cytoplasmic 1 Protein, ACTB Protein, Actb Protein, actb Protein, LOC100174883 Protein, LOC100304816 Protein, Act5C Protein, ACTG1 Protein, actb1 Protein, actb.L Protein, LOC100136352 Protein, POTEF Protein, ACTBL2 Protein, LOC109073280 Protein

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Molecular Weight: 42.6 kDa Including tag.

UniProt: P60710

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction