Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RILP Protein (AA 1-369) (His tag)

Crystallography grade RILP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Catalog No. ABIN3122531
  • Target See all RILP Proteins
    RILP (Rab Interacting Lysosomal Protein (RILP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-369
    Origin
    Mouse
    Source
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This RILP protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequence
    MEPRRAAPRL PSQASSSVGA GSAAELVYHL AGALGTELQG LARRFGPDAA AGLVPLVVRA LELLEKAAVG PAPDSLQVSA QQAEVELRRL REENQRLRQE LGSGPQEERA LLRQLKEVTD RQRDELRAHN RDLQRRSQET EALQEQLQRL LLINSELRHK LAAVQTQLRA AQDRERERQI AQDGSSQLAK EQSLEPDAAT SDDPVDTQKQ PGNLPEAVQC GFSREELKQI LQERNELKAN VFLLKEELAY FQRELLTDHR VPGLLLEAMK VAVKKQRRKI KAKMLGTPEE AESSEDEDGS WLLLSNDKED VPLVPESRIQ NFFGLWYRGE TEAPEAETSN PASSSLQKGE ETPQQPHLQP VNSPPAPNS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rilp Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product RILP Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    RILP (Rab Interacting Lysosomal Protein (RILP))
    Alternative Name
    Rilp (RILP Products)
    Synonyms
    RILP Protein, MGC154468 Protein, AI835553 Protein, Gm857 Protein, RP23-384C18.10 Protein, Rab interacting lysosomal protein Protein, Rab interacting lysosomal protein S homeolog Protein, RILP Protein, rilp.S Protein, rilp Protein, Rilp Protein
    Background
    Rab effector playing a role in late endocytic transport to degradative compartments. Involved in the regulation of lysosomal morphology and distribution. Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments. Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes (By similarity). {ECO:0000250}.
    Molecular Weight
    42.1 kDa Including tag.
    UniProt
    Q5ND29
You are here:
Support