PRDM1 Protein (AA 1-856) (Strep Tag)

Catalog No. ABIN3122687

Product Details

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-856

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PRDM1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MREAYLRCWI FSWKNVWVRP CQRLHFKTVL LQGSLLYTAL DSYSTVQAAP KSSSGSVKFQ GLAETGIMKM DMEDADMTLW TEAEFEEKCT YIVNDHPWDS GADGGTSVQA EASLPRNLLF KYAANNSKEV IGVVSKEYIP KGTRFGPLIG EVYTNDTVPK NANRKYFWRI YSREEFHHFI DGFNEEKSNW MRYVNPAHSA REQNLAACQN GMNIYFYTIK PIPANQELLV WYCRDFAERL HYPYPGELTV INLTQTESNP KQYSSEKNEL YPKSVPKREY SVKEILKLDS NPSKRKDIYR SNISPFTLEK DMDGFRKNGS PDMPFYPRVV YPIRAPLPED FLKASLAYGM ERPTYITHSP LPSSTTPSPP ASSSPEQSLK SSSPHSSPGN TVSPLAPGLP EHRDSYSYLN VSYGSEGLGS YPGYAPAPHL PPAFIPSYNA HYPKFLLPPY GISSNGLSTM NNINGINNFS LFPRLYPVYS NLLSGSSLPH PMLNPASLPS SLPTDGARRL LPPEHPKEVL IPAPHSAFSL TGAAASMKDE SSPPSGSPTA GTAATSEHVV QPKATSSVMA APSTDGAMNL IKNKRNMTGY KTLPYPLKKQ NGKIKYECNV CAKTFGQLSN LKVHLRVHSG ERPFKCQTCN KGFTQLAHLQ KHYLVHTGEK PHECQVCHKR FSSTSNLKTH LRLHSGEKPY QCKVCPAKFT QFVHLKLHKR LHTRERPHKC AQCHKSYIHL CSLKVHLKGN CPAGPAAGLP LEDLTRINEE IERFDISDNA DRLEDMEDSV DVTSMVEKEI LAVVRKEKEE TSLKVSLQRN MGNGLLSSGC SLYESSDLSL MKLPHSNPLP LVPVKVKQET VEPMDP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: Prdm1 (PRDM1 Products)

Synonyms: GB11750 Protein, CG5249 Protein, Dmel\\CG5249 Protein, blimp-1 Protein, dBlimp-1 Protein, dBlimp1 Protein, Blimp-1 Protein, Blimp1 Protein, nrd Protein, prdm1 Protein, ubo Protein, wu:fb99c12 Protein, zgc:91885 Protein, MGC52536 Protein, PRDM1 Protein, blimp1 Protein, xblimp1 Protein, prdi-bf1 Protein, BLIMP1 Protein, PRDI-BF1 Protein, ZNFPR1A1 Protein, PR/SET domain 1 Protein, probable serine/threonine-protein kinase DDB_G0282963 Protein, CG5249 gene product from transcript CG5249-RF Protein, blimp-1 Protein, PR domain containing 1a, with ZNF domain Protein, PR domain 1 S homeolog Protein, B lymphocyte induced maturation protein 1 Protein, PR domain 1 Protein, PR domain containing 1, with ZNF domain Protein, PRDM1 Protein, LOC408295 Protein, Blimp-1 Protein, CpipJ_CPIJ007425 Protein, prdm1a Protein, prdm1.S Protein, prdm1 Protein, Prdm1 Protein

Background: PR domain zinc finger protein 1 (EC 2.1.1.-) (B lymphocyte-induced maturation protein 1) (Blimp-1) (Beta-interferon gene positive regulatory domain I-binding factor) (PR domain-containing protein 1),FUNCTION: Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs (PubMed:27102484). Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection (PubMed:27102484). Binds specifically to the PRDI element in the promoter of the beta-interferon gene (By similarity). Drives the maturation of B-lymphocytes into Ig secreting cells (By similarity). Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions (PubMed:27102484). Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1 (PubMed:32741026). {ECO:0000250|UniProtKB:O75626, ECO:0000269|PubMed:27102484, ECO:0000269|PubMed:32741026}.

Molecular Weight: 95.8 kDa

UniProt: Q60636

Pathways: Regulation of Muscle Cell Differentiation