BPIFB1 Protein (AA 22-474) (His tag)
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- Target See all BPIFB1 Proteins
- BPIFB1 (BPI Fold Containing Family B, Member 1 (BPIFB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-474
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This BPIFB1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
- MNVYPPAVL NLGPEVIQKH LTQALKDHDA TAILQELPLL RAMQDKSGSI PILDSFVHTV LRYIIWMKVT SANILQLDVQ PSTYDQELVV RIPLDMVAGL NTPLIKTIVE FQMSTEVQAL IRVERSKSGP AHLNLSDCSS NESTLRLSLL HKLSFVVNSL AKNVMNLLVP ALPQIVKNHL CPVIQQAFDD MYEDFLRLTT APIALSPGAL EFGLLSPAIQ DSNILLNLKA KLLDSQARVT NWFNNSATSL METTPDRAPF SLTVRQDLVN AIVTTLVPKE ELVILLRFVI PDVARQLQMD IKEINAEAAN KLGPTQMLKI FTHSTPHIVL NEGSARAAQS VVLEVFPTNT DVRPFFSLGI EASYEAQFFT EDNRLMLNFN NVSIERIKLM ISDIKLFDPE VLKDTLTKIL EYTLLPNENG KLRTGVPMSM SKALGYEKAMW SVSKGALKLT PASSHHHHHH
- Characteristics
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The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Grade
- Crystallography grade
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BPI Fold Containing Family B, Member 1 (BPIFB1) (AA 1-484) protein (GST tag)
BPIFB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
BPI Fold Containing Family B, Member 1 (BPIFB1) (AA 22-484) protein (His tag)Crystallography grade BPIFB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
BPI Fold Containing Family B, Member 1 (BPIFB1) protein (Myc-DYKDDDDK Tag)BPIFB1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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C-terminal His-tag
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- (20 mM Hepes, pH 7.4; 50 mM NaCl; 0,1% Triton
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- BPIFB1 (BPI Fold Containing Family B, Member 1 (BPIFB1))
- Alternative Name
- Bpifb1 (BPIFB1 Products)
- Synonyms
- C20orf114 Protein, LPLUNC1 Protein, Lplunc1 Protein, C13H20orf114 Protein, RGD1563047 Protein, BPI fold containing family B member 1 Protein, BPI fold containing family B, member 1 Protein, BPIFB1 Protein, Bpifb1 Protein
- Background
- May play a role in innate immunity in mouth, nose and lungs. Binds bacterial lipopolysaccharide (LPS) and modulates the cellular responses to LPS. May be involved in formation of the left-right axis in the node of the developing embryo. {ECO:0000269|PubMed:14745963, ECO:0000269|PubMed:15028288}.
- Molecular Weight
- 51.3 kDa Including tag.
- UniProt
- Q61114
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