NELL2 Protein (AA 25-819) (His tag)

Catalog No. ABIN3122906

Product Details

Target: NELL2 (NEL-Like 2 (Chicken) (NELL2))

Protein Type: Recombinant

Protein Characteristics: AA 25-819

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This NELL2 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: LGVDPSLQID VLTELELGES TDGVRQVPGL HNGTKAFLFQ ESPRSIKAST ATAERFFQKL RNKHEFTILV TLKQIHLNSG VILSIHHLDH RYLELESSGH RNEIRLHYRS GTHRPHTEVF PYILADAKWH KLSLAFSASH LILHIDCNKI YERVVEMPST DLPLGTTFWL GQRNNAHGYF KGIMQDVHVL VMPQGFIAQC PDLNRTCPTC NDFHGLVQKI MELQDILSKT SAKLSRAEQR MNRLDQCYCE RTCTVKGTTY RESESWTDGC KNCTCLNGTI QCETLVCPAP DCPPKSAPAY VDGKCCKECK STCQFQGRSY FEGERNTVYS SSGMCVLYEC KDQTMKLVEN IGCPPLDCPE SHQIALSHSC CKVCKGYDFC SEKHTCMENS VCRNLNDRAV CSCRDGFRAL REDNAYCEDI DECAEGRHYC RENTMCVNTP GSFMCICKTG YIRIDDYSCT EHDECLTNQH NCDENALCFN TVGGHNCVCK PGYTGNGTTC KAFCKDGCRN GGACIAANVC ACPQGFTGPS CETDIDECSE GFVQCDSRAN CINLPGWYHC ECRDGYHDNG MFAPGGESCE DIDECGTGRH SCTNDTICFN LDGGYDCRCP HGKNCTGDCV HEGKVKHTGQ IWVLENDRCS VCSCQTGFVM CRRMVCDCEN PTVDLSCCPE CDPRLSSQCL HQNGETVYNS GDTWVQDCRQ CRCLQGEVDC WPLACPEVEC EFSVLPENEC CPRCVTDPCQ ADTIRNDITK TCLDEMNVVR FTGSSWIKHG TECTLCQCKN GHLCCSVDPQ CLQEL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nell2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NELL2 (NEL-Like 2 (Chicken) (NELL2))

Alternative Name: Nell2 (NELL2 Products)

Synonyms: A330108N19Rik Protein, R75516 Protein, mel91 Protein, nel Protein, NRP2 Protein, nell2 Protein, wu:fj43h11 Protein, zgc:158375 Protein, neural EGFL like 2 L homeolog Protein, NEL-like 2 Protein, neural EGFL like 2 Protein, neural EGFL like 2a Protein, nell2.L Protein, Nell2 Protein, NELL2 Protein, nell2a Protein

Background: Required for neuron survival through the modulation of MAPK pathways (By similarity). Involved in the regulation of hypothalamic GNRH secretion and the control of puberty (By similarity). {ECO:0000250|UniProtKB:Q62918}.

Molecular Weight: 89.7 kDa Including tag.

UniProt: Q61220