GUK1 Protein (AA 2-198) (His tag)

Catalog No. ABIN3123336

Product Details

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Protein Type: Recombinant

Protein Characteristics: AA 2-198

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GUK1 protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: AGPRPVVLSG PSGAGKSTLL KKLFQEHSSI FGFSVSHTTR NPRPGEEDGK DYYFVTREMM QRDIAAGDFI EHAEFSGNLY GTSKEAVRAV QAMNRICVLD VDLQGVRSIK KTDLCPIYIF VQPPSLDVLE QRLRLRNTET EESLAKRLAA ARTDMESSKE PGLFDLVIIN DDLDKAYATL KQALSEEIKK AQGTGHA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Guk1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GUK1 (Guanylate Kinase 1 (GUK1))

Alternative Name: Guk1 (GUK1 Products)

Synonyms: GMK Protein, AA409738 Protein, AL033299 Protein, AL033300 Protein, AV026611 Protein, Gmk Protein, Guk-1 Protein, guk1 Protein, zgc:73128 Protein, zgc:73217 Protein, gmk Protein, GUK1 Protein, AGK1 Protein, T11A7.23 Protein, guanylate kinase 1 Protein, DDBDRAFT_0215291 Protein, DDBDRAFT_0230100 Protein, DDB_0215291 Protein, DDB_0230100 Protein, sb:cb295 Protein, zgc:86776 Protein, guanylate kinase 1 Protein, guanylate kinase (GUK1) Protein, guanylate kinase Protein, guanylate kinase 1b Protein, guanylate kinase 1 L homeolog Protein, guanylate kinase 1a Protein, GUK1 Protein, Guk1 Protein, guk1b Protein, guk1 Protein, guk1.L Protein, GK-1 Protein, gmk Protein, gmkA Protein, Mrub_2093 Protein, Arnit_0783 Protein, Ndas_3097 Protein, Mesil_1319 Protein, Slip_0854 Protein, Olsu_0986 Protein, Acear_1451 Protein, AOR_1_2886174 Protein, guk1a Protein

Background: Essential for recycling GMP and indirectly, cGMP.

Molecular Weight: 22.7 kDa Including tag.

UniProt: Q64520

Pathways: Nucleotide Phosphorylation, ER-Nucleus Signaling, Ribonucleoside Biosynthetic Process