HDAC10 Protein (AA 1-666) (Strep Tag)

Catalog No. ABIN3123603

Product Details

Target: HDAC10 (Histone Deacetylase 10 (HDAC10))

Protein Type: Recombinant

Protein Characteristics: AA 1-666

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This HDAC10 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGTALVYHED MTATRLLWDD PECEIECPER LTAALDGLRQ RGLEERCLCL SACEASEEEL GLVHSPEYIA LVQKTQTLDK EELHALSKQY NAVYFHPDTF HCARLAAGAA LQLVDAVLTG AVHNGLALVR PPGHHSQRAA ANGFCVFNNV ALAAKHAKQK YGLQRILIVD WDVHHGQGIQ YIFNDDPSVL YFSWHRYEHG SFWPFLPESD ADAVGQGQGQ GFTVNLPWNQ VGMGNADYLA AFLHVLLPLA FEFDPELVLV SAGFDSAIGD PEGQMQATPE CFAHLTQLLQ VLAGGRICAV LEGGYHLESL AQSVCMMVQT LLGDPTPPLL GLMVPCQSAL ESIQSVQTAQ TPYWTSLQQN VAPVLSSSTH SPEERSLRLL GESPTCAVAE DSLSPLLDQL CLRPAPPICT AVASTVPGAA LCLPPGVLHQ EGSVLREETE AWARLHKSRF QDEDLATLGK ILCLLDGIMD GQIRNAIATT TALATAATLD VLIQRCLARR AQRVLCVALG QLDRPLDLAD DGRILWLNIR GKDAAIQSMF HFSTPLPQTT GGFLSLILGL VLPLAYGFQP DMVLMALGPA HGLQNAQAAL LAAMLRSPVG GRILAVVEEE SIRLLARSLA QALHGETPPS LGPFSKATPE EIQALMFLKA RLEARWKLLQ VAAPPP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HDAC10 (Histone Deacetylase 10 (HDAC10))

Alternative Name: Hdac10 (HDAC10 Products)

Synonyms: HDAC10 Protein, wu:fd19e10 Protein, zgc:55652 Protein, MGC115178 Protein, histone deacetylase 10 Protein, HD10 Protein, AW548891 Protein, Hd10 Protein, histone deacetylase 10 Protein, histone deacetylase 10 L homeolog Protein, HDAC10 Protein, hdac10 Protein, hdac10.L Protein, hda10 Protein, Hdac10 Protein

Background: Polyamine deacetylase HDAC10 (EC 3.5.1.48) (EC 3.5.1.62) (Histone deacetylase 10) (HD10),FUNCTION: Polyamine deacetylase (PDAC), which acts preferentially on N(8)-acetylspermidine, and also on acetylcadaverine and acetylputrescine. Exhibits attenuated catalytic activity toward N(1),N(8)-diacetylspermidine and very low activity, if any, toward N(1)-acetylspermidine. Histone deacetylase activity has been observed in vitro. Has also been shown to be involved in MSH2 deacetylation. The physiological relevance of protein/histone deacetylase activity is unclear and could be very weak. May play a role in the promotion of late stages of autophagy, possibly autophagosome-lysosome fusion and/or lysosomal exocytosis in neuroblastoma cells. May play a role in homologous recombination. May promote DNA mismatch repair. {ECO:0000250|UniProtKB:Q969S8}.

Molecular Weight: 72.1 kDa

UniProt: Q6P3E7