DAND5 Protein (AA 24-185) (His tag)

Catalog No. ABIN3123960

Product Details

Target: DAND5 (DAN Domain Family, Member 5 (DAND5))

Protein Type: Recombinant

Protein Characteristics: AA 24-185

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This DAND5 protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: RPGAPATPVQ SGTAINQSWT LDPLVPISAL GSWEAFLGLQ NKQQGTGELQ GGGQRVAAGV PLPLAPQEVL QETCKALSFV QVISRPGCTS ARVLNHLCFG RCSSFYIPSS DPTPVVFCNS CVPARKRWTS VTLWCGAGQL ASPRRVRIST VLVQKCQCRP KL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Dand5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DAND5 (DAN Domain Family, Member 5 (DAND5))

Alternative Name: Dand5 (DAND5 Products)

Synonyms: CER2 Protein, CERL2 Protein, CKTSF1B3 Protein, COCO Protein, CRL2 Protein, DANTE Protein, GREM3 Protein, SP1 Protein, 51-B6 Protein, cer2 Protein, cerl2 Protein, cktsf1b3 Protein, coco Protein, crl2 Protein, dante Protein, grem3 Protein, xCoco Protein, Cerl-2 Protein, Cerr2 Protein, Dte Protein, DAN domain BMP antagonist family member 5 Protein, DAN domain family member 5, BMP antagonist S homeolog Protein, DAN domain family member 5, BMP antagonist Protein, DAND5 Protein, dand5.S Protein, Dand5 Protein

Background: Seems to play a role in the correct specification of the left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-containing proteins play important roles during development, organogenesis, and tissue growth and differentiation. {ECO:0000269|PubMed:15466485}.

Molecular Weight: 18.3 kDa Including tag.

UniProt: Q76LW6