ROBO2 Protein (AA 885-1470) (His tag)

Catalog No. ABIN3124097

Product Details

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Protein Type: Recombinant

Protein Characteristics: AA 885-1470

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This ROBO2 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: YWRRKKRKGL SNYAVTFQRG DGGLMSNGSR PGLLNAGDPN YPWLADSWPA TSLPVNNSNS GPNEIGNFGR GDVLPPVPGQ GDKTATMLSD GAIYSSIDFT TKTTYNSSSQ ITQATPYATT QILHSNSIHE LAVDLPDPQW KSSVQQKTDL MGFGYSLPDQ NKGNNGGKGG KKKKTKNSSK AQKNNGSTWA NVPLPPPPVQ PLPGTELGHY AAEQENGYDS DSWCPPLPVQ TYLHQGMEDE LEEDEDRVPT PPVRGVASSP AISFGQQSTA TLTPSPREEM QPMLQAHLDE LTRAYQFDIA KQTWHIQSNT PPPQPPAPPL GYVSGALISD LETDVPDEDA DDEEEPLEIP RPLRALDQTP GSSMDNLDSS VTGKAFSSSQ RQRPTSPFST DSNTSAAQNQ SQRPRPTKKH KGGRMDPQPV LPHRREGMPD DLPPPPDPPP GQGLRQQIGL SQHSGNVENS TERKGSSLER QQAANLEDTK SSLDCPAKTV LEWQRQTQDW INSTERQEET RKAPHKQGVG SEESLVPYSK PSFPSPGGHS SSGTSSSKGS TGPRKADVLR GSHQRNANDL LDIGYVGSNS QGQFTE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Robo2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ROBO2 (Roundabout, Axon Guidance Receptor, Homolog 2 (ROBO2))

Alternative Name: Robo2 (ROBO2 Products)

Synonyms: SAX3 Protein, ROBO2 Protein, CG14347 Protein, CG14348 Protein, CG5481 Protein, CG5574 Protein, CT17326 Protein, D-Robo2 Protein, D-robo2 Protein, Dmel\\CG5481 Protein, Robo 2 Protein, Robo-2 Protein, Robo2 Protein, anon-EST:Liang-1.75 Protein, clone 1.75 Protein, dRobo-2 Protein, fus4 Protein, robo-2 Protein, robo2 Protein, unp1881 Protein, 2600013A04Rik Protein, 9430089E08Rik Protein, BB097918 Protein, D230004I22Rik Protein, mKIAA1568 Protein, sax3 Protein, roundabout guidance receptor 2 Protein, roundabout 2 Protein, roundabout, axon guidance receptor, homolog 2 (Drosophila) Protein, roundabout guidance receptor 2 S homeolog Protein, ROBO2 Protein, Robo2 Protein, robo2 Protein, robo2.S Protein

Background: Receptor for SLIT2, and probably SLIT1, which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. {ECO:0000269|PubMed:15091338}.

Molecular Weight: 64.5 kDa Including tag.

UniProt: Q7TPD3

Pathways: Regulation of Cell Size