FBXO5 Protein (AA 1-421) (Strep Tag)

Catalog No. ABIN3124183

Product Details

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Protein Type: Recombinant

Protein Characteristics: AA 1-421

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FBXO5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSRRTCSDLR RPSSCPCRLG ARTTVDGCKE ESPVLSVTMK CFNCNPDLSE LEVVKPEDSG IEASYSPVCL EPSCNDCVRN HERLSFIDSP IVGHDNKENQ RVQNTLDSSN ETEELEASRL YEDSGYSSFT QSDRDDGILI LENFRNSPQA RLLPSQSPDQ HPNKTLLPVL HFERVVCSTL KKNGKRNPKV DREMLKEVIA SGNFRLQNII GKKMGLEHLD ILAELSRRGF VHLLANILTK LSGMDLVNLS KVSRIWKKIL ENNKGAFQLY SKTMQRVIES SKLSLHATTR GYVVGRAALT CVQKSSTWAP PKKDVQIKSS SQRGQRVSTY SRHNEFVEVA KTLKNNESLK ACVRCNFPAK YDHYLERAVC KRESCQFEYC TKCLCAYHNN KDCLNGKILK ASCKVGPLPG TKKSKKNLQR L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FBXO5 (F-Box Protein 5 (FBXO5))

Alternative Name: Fbxo5 (FBXO5 Products)

Synonyms: EMI1 Protein, FBX5 Protein, Fbxo31 Protein, 2510044I10Rik Protein, C85305 Protein, Emi1 Protein, emi1 Protein, fc65h02 Protein, wu:fc65h02 Protein, wu:fe06e07 Protein, wu:fz79f03 Protein, zgc:136397 Protein, zgc:158541 Protein, fbx5 Protein, Fbxo5 Protein, ACYPI007854 Protein, fbxo5 Protein, F-box protein 5 Protein, F-box only protein 5 Protein, F-box protein 5 L homeolog Protein, F-box protein 5 S homeolog Protein, FBXO5 Protein, Fbxo5 Protein, fbxo5 Protein, fbxo5.L Protein, fbxo5.S Protein

Background: F-box only protein 5 (Early mitotic inhibitor 1),FUNCTION: Regulator of APC activity during mitotic and meiotic cell cycle (PubMed:17190794, PubMed:15526037, PubMed:16809773). During mitotic cell cycle plays a role as both substrate and inhibitor of APC-FZR1 complex (PubMed:16809773). During G1 phase, plays a role as substrate of APC-FZR1 complex E3 ligase. Then switches as an inhibitor of APC-FZR1 complex during S and G2 leading to cell-cycle commitment. As APC inhibitor, prevents the degradation of APC substrates at multiple levels: by interacting with APC and blocking access of APC substrates to the D-box co-receptor, formed by FZR1 and ANAPC10, by suppressing ubiquitin ligation and chain elongation by APC by preventing the UBE2C and UBE2S activities. Plays a role in genome integrity preservation by coordinating DNA replication with mitosis through APC inhibition in interphase to stabilize CCNA2 and GMNN in order to promote mitosis and prevent rereplication and DNA damage-induced cellular senescence (By similarity). During oocyte maturation, plays a role in meiosis through inactivation of APC-FZR1 complex. Inhibits APC through RPS6KA2 interaction that increases FBXO5 affiniy for CDC20 leading to the metaphase arrest of the second meiotic division before fertilization (PubMed:15526037). Controls entry into the first meiotic division through inactivation of APC-FZR1 complex (PubMed:17190794). Promotes migration and osteogenic differentiation of mesenchymal stem cells (By similarity). {ECO:0000250|UniProtKB:Q9UKT4, ECO:0000269|PubMed:15526037, ECO:0000269|PubMed:16809773, ECO:0000269|PubMed:17190794}.

Molecular Weight: 47.5 kDa

UniProt: Q7TSG3

Pathways: Mitotic G1-G1/S Phases