tRNA Isopentenyltransferase 1 (TRIT1) (AA 48-467) protein (His tag)

Details for Product No. ABIN3124285, Supplier: Log in to see
Protein Name
  • TRIT1
  • ipt
  • mod5
  • si:ch211-194e15.1
  • IPT
  • MOD5
  • 2310075G14Rik
  • AI314189
  • AI314635
  • AV099619
  • tRNA isopentenyltransferase 1
  • TRIT1
  • trit1
  • Trit1
Protein Characteristics
AA 48-467
1
1
1
1
Origin
Mouse (Murine)
3
1
Source
Insect Cells
1
1
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This TRIT1 protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
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Sequence GGEIVSADSM QVYEGLDIIT NKVSAQEQKM CQHHMISFVD PLVTSYTVVD FRNKATALIE DIFARDKIPI VVGGTNYYIE SLLWKVLITT KPQEMGTGKV VDRKVELEKE DGHELHKRLS QVDPEMAAKL HPHDKRKVAR SLQVFEETGI SHSEFLHRQH AEEGGGPLGG PLRFPNPCIL WLHADQAVLD ERLDKRVDDM LAAGLLEELR GFHRRYNLKN ISENSQDYQH GIFQSIGFKE FHEYLTTEGK CTPETSNQLL KKGIEALKQV TKRYARKQNR WVKNRFLSRP GPSVPPVYGL EVSDVSKWEE SVLEPALNIV QSFIQGHKPT AMPVKMAYNE SENKRSYHMC DLCDRIIIGD REWAAHLKSK SHLHQLKKRR RLDLDAVSAT GSQSNSPDCD PERIEGESSG QHNQELKASV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Trit1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Background Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). {ECO:0000250}.
Molecular Weight 48.7 kDa Including tag.
UniProt Q80UN9
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for tRNA Isopentenyltransferase 1 (TRIT1) (AA 48-467) protein (His tag) (ABIN3124285) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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